Quick Search:

uzh logo
Browse by:

Zurich Open Repository and Archive

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-35266

Cotta-Ramusino, C; Fachinetti, D; Lucca, C; Doksani, Y; Lopes, M; Sogo, J; Foiani, M (2005). Exo1 processes stalled replication forks and counteracts fork reversal in checkpoint-defective cells. Molecular Cell, 17(1):153-159.

[img] PDF - Registered users only
View at publisher


The replication checkpoint coordinates the cell cycle with DNA replication and recombination, preventing genome instability and cancer. The budding yeast Rad53 checkpoint kinase stabilizes stalled forks and replisome-fork complexes, thus preventing the accumulation of ss-DNA regions and reversed forks at collapsed forks. We searched for factors involved in the processing of stalled forks in HU-treated rad53 cells. Using the neutral-neutral two-dimensional electrophoresis technique (2D gel) and psoralen crosslinking combined with electron microscopy (EM), we found that the Exo1 exonuclease is recruited to stalled forks and, in rad53 mutants, counteracts reversed fork accumulation by generating ss-DNA intermediates. Hence, Exo1-mediated fork processing resembles the action of E. coli RecJ nuclease at damaged forks. Fork stability and replication restart are influenced by both DNA polymerase-fork association and Exo1-mediated processing. We suggest that Exo1 counteracts fork reversal by resecting newly synthesized chains and resolving the sister chromatid junctions that cause regression of collapsed forks.


136 citations in Web of Science®
135 citations in Scopus®
Google Scholar™



0 downloads since deposited on 30 Jul 2010
0 downloads since 12 months

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Molecular Cancer Research
07 Faculty of Science > Institute of Molecular Cancer Research
Dewey Decimal Classification:570 Life sciences; biology
Deposited On:30 Jul 2010 12:30
Last Modified:05 Apr 2016 14:12
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:10.1016/j.molcel.2004.11.032
PubMed ID:15629726

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page