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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-35266

Cotta-Ramusino, C; Fachinetti, D; Lucca, C; Doksani, Y; Lopes, M; Sogo, J; Foiani, M (2005). Exo1 processes stalled replication forks and counteracts fork reversal in checkpoint-defective cells. Molecular Cell, 17(1):153-159.

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Abstract

The replication checkpoint coordinates the cell cycle with DNA replication and recombination, preventing genome instability and cancer. The budding yeast Rad53 checkpoint kinase stabilizes stalled forks and replisome-fork complexes, thus preventing the accumulation of ss-DNA regions and reversed forks at collapsed forks. We searched for factors involved in the processing of stalled forks in HU-treated rad53 cells. Using the neutral-neutral two-dimensional electrophoresis technique (2D gel) and psoralen crosslinking combined with electron microscopy (EM), we found that the Exo1 exonuclease is recruited to stalled forks and, in rad53 mutants, counteracts reversed fork accumulation by generating ss-DNA intermediates. Hence, Exo1-mediated fork processing resembles the action of E. coli RecJ nuclease at damaged forks. Fork stability and replication restart are influenced by both DNA polymerase-fork association and Exo1-mediated processing. We suggest that Exo1 counteracts fork reversal by resecting newly synthesized chains and resolving the sister chromatid junctions that cause regression of collapsed forks.

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112 citations in Web of Science®
114 citations in Scopus®
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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Molecular Cancer Research
07 Faculty of Science > Institute of Molecular Cancer Research
DDC:570 Life sciences; biology
Language:English
Date:2005
Deposited On:30 Jul 2010 12:30
Last Modified:29 Nov 2013 20:52
Publisher:Elsevier
ISSN:1097-2765
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:10.1016/j.molcel.2004.11.032
PubMed ID:15629726

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