Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-35346
Petrinovic, M M; Duncan, C S; Bourikas, D; Weinman, O; Montani, L; Schroeter, A; Maerki, D; Sommer, L; Stoeckli, E T; Schwab, M E (2010). Neuronal Nogo-A regulates neurite fasciculation, branching and extension in the developing nervous system. Development, 137(15):2539-2550.
Wiring of the nervous system is a multi-step process involving complex interactions of the growing fibre with its tissue environment and with neighbouring fibres. Nogo-A is a membrane protein enriched in the adult central nervous system (CNS) myelin, where it restricts the capacity of axons to grow and regenerate after injury. During development, Nogo-A is also expressed by neurons but its function in this cell type is poorly known. Here, we show that neutralization of neuronal Nogo-A or Nogo-A gene ablation (KO) leads to longer neurites, increased fasciculation, and decreased branching of cultured dorsal root ganglion neurons. The same effects are seen with antibodies against the Nogo receptor complex components NgR and Lingo1, or by blocking the downstream effector Rho kinase (ROCK). In the chicken embryo, in ovo injection of anti-Nogo-A antibodies leads to aberrant innervation of the hindlimb. Genetic ablation of Nogo-A causes increased fasciculation and reduced branching of peripheral nerves in Nogo-A KO mouse embryos. Thus, Nogo-A is a developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||07 Faculty of Science > Institute of Molecular Life Sciences|
04 Faculty of Medicine > Brain Research Institute
|DDC:||570 Life sciences; biology|
610 Medicine & health
|Uncontrolled Keywords:||Branching, Chick, Fasciculation, Mouse, Neurite outgrowth, Nogo-A, Repulsion|
|Date:||23 June 2010|
|Deposited On:||09 Aug 2010 09:39|
|Last Modified:||27 Nov 2013 22:31|
|Publisher:||Company of Biologists|
|Citations:||Web of Science®. Times cited: 27|
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