Quick Search:

uzh logo
Browse by:

Zurich Open Repository and Archive

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-35355

Fritzsche, F R; Bode, P K; Moch, H; Kristiansen, G; Varga, Z; Bode, B (2010). Determination of the Her-2/neu gene amplification status in cytologic breast cancer specimens using automated silver-enhanced in-situ hybridization (SISH). American Journal of Surgical Pathology, 34(8):1180-1185.

Accepted Version
View at publisher


Silver-enhanced in-situ hybridization (SISH) is an emerging tool for the determination of the Her-2/neu amplification status in breast cancer. SISH is technically comparable to fluorescence in-situ hybridization (FISH) but does not require a fluorescence microscope for its interpretation. Although recent studies on histologic evaluations of SISH are promising, we aimed to evaluate its performance on 71 cytologic breast cancer specimens with the new combined Her-2/Chr17 probe. Her-2/neu status as routinely determined by FISH was available for all patients. We found SISH signals in cytologic cell blocks and smear specimens easy to evaluate in most cases. Small numbers of tumor cells and difficulties in identifying tumor cells in lymphocyte-rich backgrounds were limiting factors. Her-2/neu status, as determined by Her-2/Chr17 SISH, was basically identical to the results of the corresponding FISH. The discrepancies were mainly owing to the heterogeneity of Her-2/neu amplification in the tumor tissue. Interobserver agreement for the SISH evaluation was high (kappa value: 0.972). We conclude that Her-2/Chr17 SISH is a useful and accurate method for the evaluation of the Her-2/neu gene amplification status in cytologic breast cancer specimens, particularly in metastatic breast cancer lesions. The advantages of signal permanency and bright-field microscopic result interpretation make this technique an attractive alternative to the current FISH-based gold standard.


18 citations in Web of Science®
21 citations in Scopus®
Google Scholar™



97 downloads since deposited on 09 Aug 2010
27 downloads since 12 months

Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Institute of Surgical Pathology
Dewey Decimal Classification:610 Medicine & health
Deposited On:09 Aug 2010 14:21
Last Modified:05 Apr 2016 14:13
Publisher:Lippincott Wiliams & Wilkins
Additional Information:This is a non-final version of an article published in final form in American Journal of Surgical Pathology 2010, 34(8):1180-1185.
Publisher DOI:10.1097/PAS.0b013e3181e70e15
PubMed ID:20661016

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page