Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-36141
Gabi, M; Bullen, M E; Agarkova, I; Schmidt, D; Schoenauer, R; Brokopp, C E; Emmert, M Y; Larmagnac, A; Sannomiya, T; Weber, B; Wilhelm, M J; Vörös, J; Hoerstrup, S P (2010). Effects of small pulsed nanocurrents on cell viability in vitro and in vivo: implications for biomedical electrodes. Biomaterials, 31(33):8666-8673.
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Using a custom-built, implantable pulse generator, we studied the effects of small pulsed currents on the viability on rat aortic-derived cells (RAOC) in vitro. The pulsed currents (0.37A/m(2)) underwent apoptosis within 24h as shown by the positive staining for cleaved caspase-3 and classically apoptotic morphology. Based on these findings, we examined the effects of nanocurrents in vivo. The pulse generator was implanted subcutaneously in the rat model. The electrode|tissue interface histology revealed no difference between the active platinum surface and the neighboring control surface, however we found a large difference between electrodes that were functional during the entire experiment and non-active electrodes. These non-active electrodes showed an increase in impedance at higher frequencies 21 days post-implantation, whereas working electrodes retained their impedance value for the entire experiment. These results indicate that applied currents can reduce the impedance of implanted electrodes.
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|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > University Hospital Zurich > Clinic for Cardiovascular Surgery
04 Faculty of Medicine > University Hospital Zurich > Division of Surgical Research
04 Faculty of Medicine > Institute of Biomedical Engineering
|Dewey Decimal Classification:||170 Ethics
610 Medicine & health
|Deposited On:||15 Nov 2010 12:33|
|Last Modified:||17 Jul 2014 02:35|
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