Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-36141
Gabi, M; Bullen, M E; Agarkova, I; Schmidt, D; Schoenauer, R; Brokopp, C E; Emmert, M Y; Larmagnac, A; Sannomiya, T; Weber, B; Wilhelm, M J; Vörös, J; Hoerstrup, S P (2010). Effects of small pulsed nanocurrents on cell viability in vitro and in vivo: implications for biomedical electrodes. Biomaterials, 31(33):8666-8673.
View at publisher
Using a custom-built, implantable pulse generator, we studied the effects of small pulsed currents on the viability on rat aortic-derived cells (RAOC) in vitro. The pulsed currents (0.37A/m(2)) underwent apoptosis within 24h as shown by the positive staining for cleaved caspase-3 and classically apoptotic morphology. Based on these findings, we examined the effects of nanocurrents in vivo. The pulse generator was implanted subcutaneously in the rat model. The electrode|tissue interface histology revealed no difference between the active platinum surface and the neighboring control surface, however we found a large difference between electrodes that were functional during the entire experiment and non-active electrodes. These non-active electrodes showed an increase in impedance at higher frequencies 21 days post-implantation, whereas working electrodes retained their impedance value for the entire experiment. These results indicate that applied currents can reduce the impedance of implanted electrodes.
62 downloads since deposited on 15 Nov 2010
26 downloads since 12 months
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > University Hospital Zurich > Clinic for Cardiovascular Surgery
04 Faculty of Medicine > University Hospital Zurich > Division of Surgical Research
04 Faculty of Medicine > Institute of Biomedical Engineering
|Dewey Decimal Classification:||170 Ethics
610 Medicine & health
|Deposited On:||15 Nov 2010 12:33|
|Last Modified:||17 Jul 2014 02:35|
Users (please log in): suggest update or correction for this item
Repository Staff Only: item control page