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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-362

Veljkovic, E; Bacconi, A; Stetak, A; Hajnal, A; Stasiuk, S; Skelly, P J; Forster, I; Shoemaker, C B; Verrey, F (2004). Aromatic amino acid transporter AAT-9 of Caenorhabditis elegans localizes to neurons and muscle cells. Journal of Biological Chemistry, 279(47):49268-49273.

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The Caenorhabditis elegans genome encodes nine homologues of mammalian glycoprotein-associated amino acid transporters. Two of these C. elegans proteins (AAT-1 and AAT-3) have been shown to function as catalytic subunits (light chains) of heteromeric amino acid transporters. These proteins need to associate with a glycoprotein heavy chain subunit (ATG-2) to reach the cell surface in a manner similar to that of their mammalian homologues. AAT-1 and AAT-3 contain a cysteine residue in the second putative extracellular loop through which a disulfide bridge can form with a heavy chain. In contrast, six C. elegans members of this family (AAT-4 to AAT-9) lack such a cysteine residue. We show here that one of these transporter proteins, AAT-9, reaches the cell surface in Xenopus oocytes without an exogenous heavy chain and that it functions as an exchanger of aromatic amino acids. Two-electrode voltage clamp experiments demonstrate that AAT-9 displays a substrate-activated conductance. Immunofluorescence shows that it is expressed close to the pharyngeal bulbs within C. elegans neurons. The selective expression of an aat-9 promoter-green fluorescent protein construct in several neurons of this region and in wall muscle cells around the mouth supports and extends these localization data. Taken together, the results show that AAT-9 is expressed in excitable cells of the nematode head and pharynx in which it may provide a pathway for aromatic amino acid transport.


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Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Molecular Life Sciences
04 Faculty of Medicine > Institute of Physiology
07 Faculty of Science > Institute of Physiology
Dewey Decimal Classification:570 Life sciences; biology
Deposited On:11 Feb 2008 12:14
Last Modified:05 Apr 2016 12:13
Publisher:American Society for Biochemistry and Molecular Biology
Publisher DOI:10.1074/jbc.M404470200
PubMed ID:15364921

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