Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-3665
Baeriswyl, T; Mauti, O; Stoeckli, E T (2008). Temporal control of gene silencing by in ovo electroporation. In: Barik, S. RNAi: Design and Application. Totowa, NJ, 231-244. ISBN 978-1-58829-874-4 (Print); 978-1-59745-191-8 (Online).
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The analysis of gene function during embryonic development asks for tight temporal control of gene expression. Classic genetic tools do not allow for this, as the absence of a gene during the early stages of development will preclude its functional analysis during later stages. In contrast, RNAi technology allows one to achieve temporal control of gene silencing especially when used with oviparous animal models. In contrast to mammals, reptiles and birds are easily accessible during embryonic development. We have developed approaches to use RNAi for the analysis of gene function during nervous system development in the chicken embryo. Although the protocol given here describes a method for gene silencing in the developing spinal cord, it can easily be adapted to other parts of the developing nervous system. The combination of the easy accessibility of the chicken embryo and RNAi provides a unique opportunity for temporal and spatial control of gene silencing during development.
|Item Type:||Book Section, refereed, original work|
|Communities & Collections:||07 Faculty of Science > Institute of Molecular Life Sciences|
|DDC:||570 Life sciences; biology|
|Date:||26 March 2008|
|Deposited On:||23 Sep 2008 11:29|
|Last Modified:||01 Dec 2012 15:28|
|Series Name:||Methods in Molecular Biology|
|ISBN:||978-1-58829-874-4 (Print); 978-1-59745-191-8 (Online)|
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