Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-36724
Buschmann, J; Welti, M; Hemmi, S; Neuenschwander, P; Baltes, C; Giovanoli, P; Rudin, M; Calcagni, M (2011). 3D co-cultures of osteoblasts and endothelial cells in DegraPol foam: Histological and high field MRI analyses of pre-engineered capillary networks in bone grafts. Tissue engineering. Part C, 17(3-4):291-299.
Tissue engineering of bone grafts was addressed in a critical size model on the chick chorioallantoic membrane model (CAM assay), using DegraPol(R) (DP) foam as scaffold material. The scaffolds were seeded with cultures of human osteoblasts (OB) and human en notdo notthelial cells (EC), respectively, or with a co-culture of the two cell types (control: no cells). In vitro samples (7 days cultivation) and ex vivo CAM samples at incubation day 15 (ID 15) were analyzed by high field magnetic resonance imaging (MRI) and histology. The co-culture system performed best with respect to perfusion, as assessed by contrast-enhanced MRI using Gd-DTPA. The scaffold seeded by the co-culture supported an increased vascular ingrowth, which was confirmed by histological analysis. DP foam is a suitable scaffold for bone tissue engineering and the MRI technique allows for non-destructive and quantitative assessment of perfusion capability during early stages of bone forming constructs.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > University Hospital Zurich > Division of Surgical Research|
04 Faculty of Medicine > Institute of Pharmacology and Toxicology
04 Faculty of Medicine > Institute of Biomedical Engineering
04 Faculty of Medicine > University Hospital Zurich > Clinic for Reconstructive Surgery
|DDC:||570 Life sciences; biology|
610 Medicine & health
|Deposited On:||05 Nov 2010 09:13|
|Last Modified:||23 Nov 2012 14:52|
|Publisher:||Mary Ann Liebert|
|Additional Information:||This is a copy of an article published in the Tissue Engineering Part A © 2011 copyright Mary Ann Liebert, Inc.; Tissue Engineering Part A is available online at: http://www.liebertonline.com.|
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