Quick Search:

is currently disabled due to reindexing of the ZORA database. Please use Advanced Search.
uzh logo
Browse by:
bullet
bullet
bullet
bullet

Zurich Open Repository and Archive 

Künzler, M; Bleuler-Martinez, S; Butschi, A; Garbani, M; Lüthy, P; Hengartner, M O; Aebi, M (2010). Biotoxicity assays for fruiting body lectins and other cytoplasmic proteins. Methods in Enzymology, 480:141-50.

Full text not available from this repository.

Abstract

Recent studies suggest that a specific class of fungal lectins, commonly referred to as fruiting body lectins, play a role as effector molecules in the defense of fungi against predators and parasites. Hallmarks of these fungal lectins are their specific expression in reproductive structures, fruiting bodies, and/or sclerotia and their synthesis on free ribosomes in the cytoplasm. Fruiting body lectins are released upon damage of the fungal cell and bind to specific carbohydrate structures of predators and parasites, which leads to deterrence, inhibition of growth, and development or even killing of these organisms. Here, we describe assays to assess the toxicity of such lectins and other cytoplasmic proteins toward three different model organisms: the insect Aedes aegypti, the nematode Caenorhabditis elegans, and the amoeba Acanthamoeba castellanii. All three assays are based on heterologous expression of the examined proteins in the cytoplasm of Escherichia coli and feeding of these recombinant bacteria to omnivorous and bacterivorous organisms.

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Molecular Life Sciences
DDC:570 Life sciences; biology
Language:English
Date:2010
Deposited On:05 Nov 2010 09:58
Last Modified:27 Nov 2013 17:18
Publisher:Elsevier
ISSN:0076-6879
Publisher DOI:10.1016/S0076-6879(10)80007-2
PubMed ID:20816208
Citations:Web of Science®. Times Cited: 7
Google Scholar™

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page