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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-36733

Cabello, J; Neukomm, L J; Günesdogan, U; Burkart, K; Charette, S J; Lochnit, G; Hengartner, M O; Schnabel, R (2010). The Wnt pathway controls cell death engulfment, spindle orientation, and migration through CED-10/Rac. PLoS Biology, 8(2):e1000297.

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Abstract

Wnt signalling pathways have extremely diverse functions in animals, including induction of cell fates or tumours, guidance of cell movements during gastrulation, and the induction of cell polarity. Wnt can induce polar changes in cellular morphology by a remodelling of the cytoskeleton. However, how activation of the Frizzled receptor induces cytoskeleton rearrangement is not well understood. We show, by an in depth 4-D microscopy analysis, that the Caenorhabditis elegans Wnt pathway signals to CED-10/Rac via two separate branches to regulate modulation of the cytoskeleton in different cellular situations. Apoptotic cell clearance and migration of the distal tip cell require the MOM-5/Fz receptor, GSK-3 kinase, and APC/APR-1, which activate the CED-2/5/12 branch of the engulfment machinery. MOM-5 (Frizzled) thus can function as an engulfment receptor in C. elegans. Our epistatic analyses also suggest that the two partially redundant signalling pathways defined earlier for engulfment may act in a single pathway in early embryos. By contrast, rearrangement of mitotic spindles requires the MOM-5/Fz receptor, GSK-3 kinase, and beta-catenins, but not the downstream factors LIT-1/NLK or POP-1/Tcf. Taken together, our results indicate that in multiple developmental processes, CED-10/Rac can link polar signals mediated by the Wnt pathway to rearrangements of the cytoskeleton.

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Molecular Life Sciences
DDC:570 Life sciences; biology
Language:English
Date:2010
Deposited On:05 Nov 2010 08:27
Last Modified:27 Nov 2013 17:22
Publisher:Public Library of Science
ISSN:1544-9173
Publisher DOI:10.1371/journal.pbio.1000297
PubMed ID:20126385
Citations:Web of Science®. Times Cited: 26
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Scopus®. Citation Count: 31

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