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Scattered light fluorescence microscopy: imaging through turbid layers


Vellekoop, I M; Aegerter, C M (2010). Scattered light fluorescence microscopy: imaging through turbid layers. Optics Letters, 35(8):1245-1247.

Abstract

A major limitation of any type of microscope is the penetration depth in turbid tissue. Here, we demonstrate a fundamentally novel kind of fluorescence microscope that images through optically thick turbid layers. The microscope uses scattered light, rather than light propagating along a straight path, for imaging with subwavelength resolution. Our method uses constructive interference to focus scattered laser light through the turbid layer. Microscopic fluorescent structures behind the layer were imaged by raster scanning the focus.

Abstract

A major limitation of any type of microscope is the penetration depth in turbid tissue. Here, we demonstrate a fundamentally novel kind of fluorescence microscope that images through optically thick turbid layers. The microscope uses scattered light, rather than light propagating along a straight path, for imaging with subwavelength resolution. Our method uses constructive interference to focus scattered laser light through the turbid layer. Microscopic fluorescent structures behind the layer were imaged by raster scanning the focus.

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83 citations in Web of Science®
93 citations in Scopus®
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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Physics Institute
Special Collections > SystemsX.ch
Special Collections > SystemsX.ch > Research, Technology and Development Projects > WingX
Dewey Decimal Classification:570 Life sciences; biology
530 Physics
Language:English
Date:2010
Deposited On:01 Nov 2010 14:56
Last Modified:05 Apr 2016 14:18
Publisher:Optical Society of America (OSA)
ISSN:0146-9592
Additional Information:This paper was published in Optics Letters and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://dx.doi.org/10.1364/OL.35.001245. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.
Publisher DOI:https://doi.org/10.1364/OL.35.001245
PubMed ID:20410981

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