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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-38389

Lê, K A; Faeh, D; Stettler, R; Debard, C; Loizon, E; Vidal, H; Boesch, C; Ravussin, E; Tappy, L (2008). Effects of four-week high-fructose diet on gene expression in skeletal muscle of healthy men. Diabetes & Metabolism, 34(1):82-85.

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Abstract

AIMS: A high-fructose diet (HFrD) may play a role in the obesity and metabolic disorders epidemic. In rodents, HFrD leads to insulin resistance and ectopic lipid deposition. In healthy humans, a four-week HFrD alters lipid homoeostasis, but does not affect insulin sensitivity or intramyocellular lipids (IMCL). The aim of this study was to investigate whether fructose may induce early molecular changes in skeletal muscle prior to the development of whole-body insulin resistance. METHODS: Muscle biopsies were taken from five healthy men who had participated in a previous four-week HFrD study, during which insulin sensitivity (hyperinsulinaemic euglycaemic clamp), and intrahepatocellular lipids and IMCL were assessed before and after HFrD. The mRNA concentrations of 16 genes involved in lipid and carbohydrate metabolism were quantified before and after HFrD by real-time quantitative PCR. RESULTS: HFrD significantly (P<0.05) increased stearoyl-CoA desaturase-1 (SCD-1) (+50%). Glucose transporter-4 (GLUT-4) decreased by 27% and acetyl-CoA carboxylase-2 decreased by 48%. A trend toward decreased peroxisomal proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) was observed (-26%, P=0.06). All other genes showed no significant changes. CONCLUSION: HFrD led to alterations of SCD-1, GLUT-4 and PGC-1alpha, which may be early markers of insulin resistance.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Social and Preventive Medicine
DDC:610 Medicine & health
Language:English
Date:2008
Deposited On:18 Feb 2011 11:12
Last Modified:14 Dec 2013 14:23
Publisher:Elsevier
ISSN:1262-3636
Free access at:PubMed ID. An embargo period may apply.
Publisher DOI:10.1016/j.diabet.2007.08.004
PubMed ID:18063403
Citations:Web of Science®. Times Cited: 18
Google Scholar™
Scopus®. Citation Count: 18

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