Quick Search:

uzh logo
Browse by:

Zurich Open Repository and Archive

Maintenance: Tuesday, 5.7.2016, 07:00-08:00

Maintenance work on ZORA and JDB on Tuesday, 5th July, 07h00-08h00. During this time there will be a brief unavailability for about 1 hour. Please be patient.

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-391

Geisler, R; Rauch, G J; Geiger-Rudolph, S; Albrecht, A J; van Bebber, F; Berger, A; Busch-Nentwich, E; Dahm, R; Dekens, M P S; Dooley, C; Elli, A F; Gehring, I; Geiger, H; Geisler, M; Glaser, S; Holley, S; Huber, M; Kerr, A; Kirn, A; Knirsch, M; Konantz, M; Küchler, A M; Maderspacher, F; Neuhauss, S C F; Nicolson, T; Ober, E A; Praeg, E; Ray, R; Rentzsch, B; Rick, J M; Rief, E; Schauerte, H E; Schepp, C P; Schönberger, U; Schonthaler, H B; Seiler, C; Sidi, S; Söllner, C; Wehner, A; Weiler, C; Nüsslein-Volhard, C (2007). Large-scale mapping of mutations affecting zebrafish development. BMC Genomics, 8:11.

View at publisher


BACKGROUND: Large-scale mutagenesis screens in the zebrafish employing the mutagen ENU have isolated several hundred mutant loci that represent putative developmental control genes. In order to realize the potential of such screens, systematic genetic mapping of the mutations is necessary. Here we report on a large-scale effort to map the mutations generated in mutagenesis screening at the Max Planck Institute for Developmental Biology by genome scanning with microsatellite markers. RESULTS: We have selected a set of microsatellite markers and developed methods and scoring criteria suitable for efficient, high-throughput genome scanning. We have used these methods to successfully obtain a rough map position for 319 mutant loci from the Tübingen I mutagenesis screen and subsequent screening of the mutant collection. For 277 of these the corresponding gene is not yet identified. Mapping was successful for 80 % of the tested loci. By comparing 21 mutation and gene positions of cloned mutations we have validated the correctness of our linkage group assignments and estimated the standard error of our map positions to be approximately 6 cM. CONCLUSION: By obtaining rough map positions for over 300 zebrafish loci with developmental phenotypes, we have generated a dataset that will be useful not only for cloning of the affected genes, but also to suggest allelism of mutations with similar phenotypes that will be identified in future screens. Furthermore this work validates the usefulness of our methodology for rapid, systematic and inexpensive microsatellite mapping of zebrafish mutations.


39 citations in Web of Science®
36 citations in Scopus®
Google Scholar™



637 downloads since deposited on 11 Feb 2008
147 downloads since 12 months

Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Molecular Life Sciences
Dewey Decimal Classification:570 Life sciences; biology
Deposited On:11 Feb 2008 12:14
Last Modified:05 Apr 2016 12:14
Publisher:BioMed Central
Publisher DOI:10.1186/1471-2164-8-11
Official URL:http://www.biomedcentral.com/content/pdf/1471-2164-8-11.pdf
PubMed ID:17212827

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page