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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-39781

Franchini, M; Zini, E; Osto, M; Jablonski, K; Kaufmann, K; Lutz, T A; Reusch, C E; Ackermann, M (2010). Feline pancreatic islet-like clusters and insulin producing cells express functional Toll-like receptors (TLRs). Veterinary Immunology and Immunopathology, 138(1-2):70-78.

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Toll-like receptors (TLRs) are cellular receptors that recognize molecules derived from pathogens, endogenous molecules generated after cellular stress, and free fatty acids. TLR activation leads to a proinflammatory reaction that is fundamental in the initiation of an innate immune response and subsequent adaptive responses but also can damage tissues. TLRs are not only expressed within the immune system, but also in most other organ systems including the pancreas. TLR4 is expressed in pancreatic Beta-cells of rodents and humans and its stimulation affects insulin secretion in response to glucose. A low-grade inflammation is often associated with disturbed performance of Beta-cells and insulin resistance, the cardinal metabolic event of type-2 diabetes. Feline diabetes mellitus shares many similarities with type-2 diabetes in humans. Our objective was to elucidate the role of TLRs in feline pancreatic islets and islet-like clusters (ILC) that consist of islets with their adjacent tissue.Wetested whether TLRs are triggered by their agonists and lead to the expression of inflammatory cytokines. Weconfirmed the expression of allknown feline TLRs in pancreas and ILC. Furthermore, stimulation with TLR agonists increased IL-6 mRNA and protein content and the expression of other proinflammatory cytokines indicating
a clear proinflammatory response. The reactivity to TLR ligands was stronger in Beta-cell enriched populations obtained after sorting by FACS indicating that inflammatory
stimuli can also be generated within Beta-cells. We conclude that the microenvironment of feline Beta-cells harbor the potential for inflammatory reactions, that can be initiated by molecules released from bacteria or viruses or other molecules recognized by TLRs. Therefore infections associated with bacteriemia and viremia can induce inflammation in islets and damage the endocrine pancreatic tissue.

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Veterinary Physiology
05 Vetsuisse Faculty > Veterinary Clinic > Department of Small Animals > Clinic for Small Animal Internal Medicine
05 Vetsuisse Faculty > Institute of Virology
DDC:570 Life sciences; biology
630 Agriculture
Deposited On:27 Jan 2011 08:36
Last Modified:27 Nov 2013 20:37
Publisher DOI:10.1016/j.vetimm.2010.07.001
Citations:Web of Science®. Times Cited: 3
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Scopus®. Citation Count: 5

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