Keller, P M; Rampini, S K; Bloemberg, G V (2010). Detection of a mixed infection in a culture-negative brain abscess by broad-spectrum bacterial 16S rRNA gene PCR. Journal of Clinical Microbiology, 48(6):2250-2252.
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We describe the identification of two bacterial pathogens from a culture-negative brain abscess by the use of broad-spectrum 16S rRNA gene PCR. Simultaneous detection of Fusobacterium nucleatum and Porphyromonas endodontalis was possible due to a 24-bp length difference of their partially amplified 16S rRNA genes, which allowed separation by high-resolution polyacrylamide gel electrophoresis.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > University Hospital Zurich > Clinic and Policlinic for Internal Medicine|
04 Faculty of Medicine > Institute of Medical Microbiology
|DDC:||570 Life sciences; biology|
610 Medicine & health
|Deposited On:||24 Jan 2011 16:15|
|Last Modified:||27 Nov 2013 19:29|
|Publisher:||American Society for Microbiology|
|Free access at:||PubMed ID. An embargo period may apply.|
|Citations:||Web of Science®. Times cited: 6|
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