Keller, P M; Rampini, S K; Bloemberg, G V (2010). Detection of a mixed infection in a culture-negative brain abscess by broad-spectrum bacterial 16S rRNA gene PCR. Journal of Clinical Microbiology, 48(6):2250-2252.
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Abstract
We describe the identification of two bacterial pathogens from a culture-negative brain abscess by the use of broad-spectrum 16S rRNA gene PCR. Simultaneous detection of Fusobacterium nucleatum and Porphyromonas endodontalis was possible due to a 24-bp length difference of their partially amplified 16S rRNA genes, which allowed separation by high-resolution polyacrylamide gel electrophoresis.
| Item Type: | Journal Article, refereed, original work |
|---|---|
| Communities & Collections: | 04 Faculty of Medicine > University Hospital Zurich > Clinic and Policlinic for Internal Medicine 04 Faculty of Medicine > Institute of Medical Microbiology |
| DDC: | 570 Life sciences; biology 610 Medicine & health |
| Language: | English |
| Date: | 2010 |
| Deposited On: | 24 Jan 2011 16:15 |
| Last Modified: | 23 Nov 2012 16:14 |
| Publisher: | American Society for Microbiology |
| ISSN: | 0095-1137 |
| Free access at: | PubMed ID. An embargo period may apply. |
| Publisher DOI: | 10.1128/JCM.01922-09 |
| PubMed ID: | 20392909 |
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