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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-3985

Casson, N; Entenza, J M; Borel, N; Pospischil, A; Greub, G (2008). Murine model of pneumonia caused by Parachlamydia acanthamoebae. Microbial Pathogenesis, 45(2):92-97.

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The role of Parachlamydia acanthamoebae as an agent of pneumonia is suggested by sero-epidemiological studies, molecular surveys and by the permissivity of macrophages, lung fibroblasts and pneumocytes to this obligate intracellular bacteria. We thus developed a murine model of pneumonia due to Parachlamydia. Mice were inoculated intratracheally with Parachlamydia acanthamoebae. Pneumonia-associated mortality was of 50% 5 days post-inoculation. Lungs histopathology was characterized by purulent and interstitial pneumonia. The presence of Parachlamydia in the lesions was demonstrated by PCR, immunohistochemistry and electron microscopy. Moreover, living Parachlamydia could be recovered from the lungs of infected mice using amoebal co-culture. All control mice inoculated with heat-inactivated bacteria were free of symptoms and survived. Thus, we demonstrated that Parachlamydia induce a severe pneumonia in mice. This animal model, which confirms the third and fourth Koch postulates, may be suitable to test in vivo efficient therapeutic regimens against Parachlamydia.


18 citations in Web of Science®
21 citations in Scopus®
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1 download since deposited on 25 Nov 2008
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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Veterinary Pathology
Dewey Decimal Classification:570 Life sciences; biology
Uncontrolled Keywords:Chlamydiae Parachlamydiaceae Mouse model Lower respiratory tract infection
Date:August 2008
Deposited On:25 Nov 2008 20:28
Last Modified:05 Apr 2016 12:28
Publisher:Academic Press
Publisher DOI:10.1016/j.micpath.2008.04.003
PubMed ID:18502092

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