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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-4014

Waerzeggers, Y; Klein, M; Miletic, H; Himmelreich, U; Li, H; Monfared, P; Herrlinger, U; Hoehn, M; Coenen, H H; Weller, M; Winkeler, A; Jacobs, A H (2008). Multimodal imaging of neural progenitor cell fate in rodents. Molecular Imaging, 7(2):77-91.

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Abstract

For clinical application of stem cell-based therapies, noninvasive detection of applied stem cells is of high importance. We report on the feasibility of detecting implanted neural progenitor cells (NPCs) noninvasively and follow their fate and functional status by sequential multimodal molecular imaging and reporter gene technology. We investigated C17.2 cells stably expressing herpes simplex virus type 1-thymidine kinase (HSV-1-tk) and green fluorescent protein (gfp) (C17.2-tkIRESgfp = C17.2-TIG) or HSV-1-tk, gfp, and firefly luciferase (luc) (C17.2-lucIREStkgfp = C17.2-LITG) and determined the detection sensitivity of positron emission tomography (PET) and bioluminescence imaging (BLI) for these cells in culture and in vivo in subcutaneous and intracranial glioma models. In addition, PET and BLI were used to further investigate and follow the fate of implanted C17.2-LITG cells in an intracranial glioma model. We show that both imaging modalities are sensitive in detecting reporter gene expressing NPCs; however, PET, by the use of 9-[4-[(18)F]fluoro-3-hydroxymethyl)butyl]guanine ([(18)F]FHBG), detects NPCs only at sites of disrupted blood-brain barrier. Furthermore, both imaging modalities can be used to detect stem cell fate and migration and indicate excessive proliferation and aberrant migration. In conclusion, multimodal imaging can be used for longitudinal noninvasive monitoring of grafted NPCs in rodents.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Clinic for Neurology
DDC:610 Medicine & health
Language:English
Date:April 2008
Deposited On:21 Nov 2008 14:07
Last Modified:27 Nov 2013 20:15
Publisher:MIT Press
ISSN:1535-3508
Additional Information:Copyright: MIT Press
Publisher DOI:10.2310/7290.2008.0010
PubMed ID:18706290
Citations:Web of Science®. Times Cited: 31
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