Quick Search:

uzh logo
Browse by:
bullet
bullet
bullet
bullet

Zurich Open Repository and Archive

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-4014

Waerzeggers, Y; Klein, M; Miletic, H; Himmelreich, U; Li, H; Monfared, P; Herrlinger, U; Hoehn, M; Coenen, H H; Weller, M; Winkeler, A; Jacobs, A H (2008). Multimodal imaging of neural progenitor cell fate in rodents. Molecular Imaging, 7(2):77-91.

[img]
Preview
Accepted Version
PDF
4MB

View at publisher

Abstract

For clinical application of stem cell-based therapies, noninvasive detection of applied stem cells is of high importance. We report on the feasibility of detecting implanted neural progenitor cells (NPCs) noninvasively and follow their fate and functional status by sequential multimodal molecular imaging and reporter gene technology. We investigated C17.2 cells stably expressing herpes simplex virus type 1-thymidine kinase (HSV-1-tk) and green fluorescent protein (gfp) (C17.2-tkIRESgfp = C17.2-TIG) or HSV-1-tk, gfp, and firefly luciferase (luc) (C17.2-lucIREStkgfp = C17.2-LITG) and determined the detection sensitivity of positron emission tomography (PET) and bioluminescence imaging (BLI) for these cells in culture and in vivo in subcutaneous and intracranial glioma models. In addition, PET and BLI were used to further investigate and follow the fate of implanted C17.2-LITG cells in an intracranial glioma model. We show that both imaging modalities are sensitive in detecting reporter gene expressing NPCs; however, PET, by the use of 9-[4-[(18)F]fluoro-3-hydroxymethyl)butyl]guanine ([(18)F]FHBG), detects NPCs only at sites of disrupted blood-brain barrier. Furthermore, both imaging modalities can be used to detect stem cell fate and migration and indicate excessive proliferation and aberrant migration. In conclusion, multimodal imaging can be used for longitudinal noninvasive monitoring of grafted NPCs in rodents.

Citations

32 citations in Web of Science®
9 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

183 downloads since deposited on 21 Nov 2008
25 downloads since 12 months

Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Clinic for Neurology
DDC:610 Medicine & health
Language:English
Date:April 2008
Deposited On:21 Nov 2008 13:07
Last Modified:27 Nov 2013 19:15
Publisher:MIT Press
ISSN:1535-3508
Additional Information:Copyright: MIT Press
Publisher DOI:10.2310/7290.2008.0010
PubMed ID:18706290

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page