Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-40378
Kraehenbuehl, T P; Stauber, M; Ehrbar, M; Weber, F; Hall, H; Müller, R (2010). Effects of muCT radiation on tissue engineered bone-like constructs. Biomedizinische Technik. Biomedical Engineering, 55(4):245-250.
High-resolution, non-destructive imaging with micro-computed tomography (muCT) enables in situ monitoring of tissue engineered bone constructs. However, it remains controversial, if the locally imposed X-ray dose affects bone development and thus could influence the results. Here, we developed a model system for muCT monitoring of tissue engineered bone-like constructs. We examined the in vitro effects of high-resolution muCT imaging on the cellular level by using pre-osteoblastic MC3T3-E1 cells embedded into three-dimensional collagen type I matrices. We found no significantly reduced cell survival 2 h after irradiation with a dose of 1.9 Gy. However, 24 h post-irradiation, cell survival was significantly decreased by 15% compared to non-irradiated samples. The highest dose of 7.6 Gy decreased survival of the pre-osteoblastic MC3T3-E1 cells by around 40% at 2 days post-irradiation. No significant increase of alkaline phosphatase (ALP) activity at 2 days post-irradiation was found with a dose of 1.9 Gy. However, ALP activity was significantly decreased after 7 days. Using our model system, the results indicate that muCT imaging with doses as low as 1.9 Gy, which is required to obtain a reasonable image quality, can induce irreparable damages on the cellular level.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > University Hospital Zurich > Division of Surgical Research|
04 Faculty of Medicine > University Hospital Zurich > Clinic for Obstetrics
04 Faculty of Medicine > Center for Dental Medicine > Clinic for Cranio-Maxillofacial Surgery
|DDC:||610 Medicine & health|
|Deposited On:||05 Jan 2011 14:36|
|Last Modified:||28 Nov 2013 02:04|
|Free access at:||PubMed ID. An embargo period may apply.|
|Citations:||Web of Science®. Times cited: 1|
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