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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-41176

Mathis, C; Schröter, A; Thallmair, M; Schwab, M E (2010). Nogo-a regulates neural precursor migration in the embryonic mouse cortex. Cerebral Cortex, 20(10):2380-2390.

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Abstract

Although Nogo-A has been intensively studied for its inhibitory effect on axonal regeneration in the adult central nervous system, little is known about its function during brain development. In the embryonic mouse cortex, Nogo-A is expressed by radial precursor/glial cells and by tangentially migrating as well as postmigratory neurons. We studied radially migrating neuroblasts in wild-type and Nogo-A knockout (KO) mouse embryos. In vitro analysis showed that Nogo-A and its receptor components NgR, Lingo-1, TROY, and p75 are expressed in cells emigrating from embryonic forebrain-derived neurospheres. Live imaging revealed an increased cell motility when Nogo-A was knocked out or blocked with antibodies. Antibodies blocking NgR or Lingo-1 showed the same motility-enhancing effect supporting a direct role of surface Nogo-A on migration. Bromodeoxyuridine (BrdU) labeling of embryonic day (E)15.5 embryos demonstrated that Nogo-A influences the radial migration of neuronal precursors. At E17.5, the normal transient accumulation of radially migrating precursors within the subventricular zone was not detectable in the Nogo-A KO mouse cortex. At E19, migration to the upper cortical layers was disturbed. These findings suggest that Nogo-A and its receptor complex play a role in the interplay of adhesive and repulsive cell interactions in radial migration during cortical development.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Brain Research Institute
DDC:570 Life sciences; biology
610 Medicine & health
Language:English
Date:October 2010
Deposited On:05 Jan 2011 09:20
Last Modified:07 Jan 2014 00:08
Publisher:Oxford University Press
ISSN:1047-3211
Free access at:PubMed ID. An embargo period may apply.
Publisher DOI:10.1093/cercor/bhp307
PubMed ID:20093372
Citations:Web of Science®. Times Cited: 23
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