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Schiestl, C; Biedermann, T; Braziulis, E; Hartmann-Fritsch, F; Böttcher-Haberzeth, S; Arras, M; Cesarovic, N; Nicolls, F; Linti, C; Reichmann, E; Meuli, M (2011). Skingineering II: transplantation of large-scale laboratory-grown skin analogues in a new pig model. Pediatric Surgery International, 27(3):249-254.

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Abstract

BACKGROUND: Tissue engineering of skin with near-normal anatomy is an intriguing novel strategy to attack the still unsolved problem of how to ideally cover massive full-thickness skin defects. After successful production of large, pig cell-derived skin analogues, we now aim at developing an appropriate large animal model for transplantation studies. MATERIALS AND METHODS: In four adult Swiss pigs, full-thickness skin defects, measuring 7.5 × 7.5 cm, were surgically created and then shielded against the surrounding skin by a new, self-designed silicone chamber. In two animals each, Integra dermal regeneration templates or cultured autologous skin analogues, respectively, were applied onto the wound bed. A sophisticated shock-absorbing dressing was applied for the ensuing 3 weeks. Results were documented photographically and histologically. RESULTS: All animals survived uneventfully. Integra healed in perfectly, while the dermo-epidermal skin analogues showed complete take of the dermal compartment but spots of missing epidermis. The chamber proved effective in precluding ("false positive") healing from the wound edges and the special dressing efficiently kept the operation site intact and clean for the planned 3 weeks. CONCLUSION: We present a novel and valid pig model permitting both transplantation of large autologous, laboratory-engineered skin analogues and also keeping the site of intervention undisturbed for at least three postoperative weeks. Hence, the model will be used for experiments testing whether such large skin analogues can restore near-normal skin, particularly in the long term. If so, clinical application can be envisioned.

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6 citations in Web of Science®
6 citations in Scopus®
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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Division of Surgical Research
04 Faculty of Medicine > University Children's Hospital Zurich > Clinic for Surgery
DDC:610 Medicine & health
Language:English
Date:2011
Deposited On:31 Jan 2011 10:22
Last Modified:27 Nov 2013 22:04
Publisher:Springer
ISSN:0179-0358
Publisher DOI:10.1007/s00383-010-2792-1
PubMed ID:21069348

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