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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-41726

Bublin, M; Pfister, M; Radauer, C; Oberhuber, C; Bulley, S; Dewitt, A M; Lidholm, J; Reese, G; Vieths, S; Breiteneder, H; Hoffmann-Sommergruber, K; Ballmer-Weber, B K (2010). Component-resolved diagnosis of kiwifruit allergy with purified natural and recombinant kiwifruit allergens. Journal of Allergy and Clinical Immunology, 125(3):687-694, 694.e1.

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Abstract

BACKGROUND: Kiwifruit is one of the most common causes of food allergic reactions. Component-resolved diagnostics may enable significantly improved detection of sensitization to kiwifruit.

OBJECTIVE: To evaluate the use of individual allergens for component-resolved in vitro diagnosis of kiwifruit allergy.

METHODS: Thirty patients with a positive double-blind placebo-controlled food challenge to kiwifruit, 10 atopic subjects with negative open provocation to kiwifruit, and 5 nonatopic subjects were enrolled in the study. Specific IgE to 7 individual allergens (nAct d 1-5 and rAct d 8-9) and allergen extracts was measured by ImmunoCAP.

RESULTS: The diagnostic sensitivities of the commercial extract and of the sum of single allergens were 17% and 77%, respectively, whereas diagnostic specificities were 100% and 30%. A combination of the kiwi allergens Act d 1, Act d 2, Act d 4, and Act d 5 gave a diagnostic sensitivity of 40%, whereas diagnostic specificity remained high (90%). Exclusion of the Bet v 1 homolog recombinant (r) Act d 8 and profilin rAct d 9 from this allergen panel reduced sensitivity to 50% but increased specificity to 40%. Kiwifruit-monosensitized patients reacted more frequently (P < .001) with Act d 1 than polysensitized patients, whereas the latter group reacted more frequently with rAct d 8 (P = .004).

CONCLUSION: Use of single kiwifruit allergen ImmunoCAP increases the quantitative test performance and diagnostic sensitivity compared with the commercial extract. Bet v 1 homolog and profilin are important allergens in pollen-related kiwifruit allergy, whereas actinidin is important in monoallergy to kiwifruit, in which symptoms are often more severe.

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39 citations in Web of Science®
48 citations in Scopus®
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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Dermatology Clinic
DDC:610 Medicine & health
Language:English
Date:2010
Deposited On:08 Jan 2011 16:53
Last Modified:04 Dec 2013 01:08
Publisher:Elsevier
ISSN:0091-6749
Publisher DOI:10.1016/j.jaci.2009.10.017
PubMed ID:20061012

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