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Activation of the inflammasome by amorphous silica and TiO(2) nanoparticles in murine dendritic cells


Winter, M; Beer, H D; Hornung, V; Krämer, U; Schins, R P F; Förster, I (2011). Activation of the inflammasome by amorphous silica and TiO(2) nanoparticles in murine dendritic cells. Nanotoxicology, 5(3):326-340.

Abstract

Abstract Nanomaterials are increasingly used in various food applications. In particular, nanoparticulate amorphous SiO(2) is already contained, e.g., in spices. Since intestinal dendritic cells (DC) could be critical targets for ingested particles, we compared the in vitro effects of amorphous silica nanoparticles with fine crystalline silica, and micron-sized with nano-sized TiO(2) particles on DC. TiO(2)- and SiO(2)-nanoparticles, as well as crystalline silica led to an upregulation of MHC-II, CD80, and CD86 on DC. Furthermore, these particles activated the inflammasome, leading to significant IL-1β-secretion in wild-type (WT) but not Caspase-1- or NLRP3-deficient mice. Silica nanoparticles and crystalline silica induced apoptosis, while TiO(2) nanoparticles led to enhanced production of reactive oxygen species (ROS). Since amorphous silica and TiO(2) nanoparticles had strong effects on the activation-status of DC, we suggest that nanoparticles, used as food additives, should be intensively studied in vitro and in vivo, to ensure their safety for the consumer.

Abstract Nanomaterials are increasingly used in various food applications. In particular, nanoparticulate amorphous SiO(2) is already contained, e.g., in spices. Since intestinal dendritic cells (DC) could be critical targets for ingested particles, we compared the in vitro effects of amorphous silica nanoparticles with fine crystalline silica, and micron-sized with nano-sized TiO(2) particles on DC. TiO(2)- and SiO(2)-nanoparticles, as well as crystalline silica led to an upregulation of MHC-II, CD80, and CD86 on DC. Furthermore, these particles activated the inflammasome, leading to significant IL-1β-secretion in wild-type (WT) but not Caspase-1- or NLRP3-deficient mice. Silica nanoparticles and crystalline silica induced apoptosis, while TiO(2) nanoparticles led to enhanced production of reactive oxygen species (ROS). Since amorphous silica and TiO(2) nanoparticles had strong effects on the activation-status of DC, we suggest that nanoparticles, used as food additives, should be intensively studied in vitro and in vivo, to ensure their safety for the consumer.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Dermatology Clinic
Dewey Decimal Classification:610 Medicine & health
Language:English
Date:2011
Deposited On:07 Jan 2011 15:46
Last Modified:05 Apr 2016 14:33
Publisher:Informa Healthcare
ISSN:1743-5390
Publisher DOI:https://doi.org/10.3109/17435390.2010.506957
Official URL:http://informahealthcare.com/doi/full/10.3109/17435390.2010.506957
PubMed ID:20846021
Permanent URL: https://doi.org/10.5167/uzh-41842

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