Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-42
Bahadori, R; Biehlmaier, O; Zeitz, C; Labhart, T; Makhankov, Y V; Forster, U; Gesemann, M; Berger, W; Neuhauss, S C F (2006). Nyctalopin is essential for synaptic transmission in the cone dominated zebrafish retina. European Journal of Neuroscience, 24(6):1664-1674.
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The first synapse in the vertebrate visual system is the photoreceptor synapse between rod and cone photoreceptors and the second-order bipolar cells. Although mutations in the nyctalopin gene (NYX) in humans lead to congenital stationary night blindness (CSNB1), affecting synaptic transmission between both types of photoreceptors and ON-bipolar cells, the function of nyctalopin in cone-dominant animal models has not been studied. Because the larval zebrafish retina is cone-dominant, we isolated the zebrafish nyx ortholog and raised a polyclonal antibody against the protein. Nyctalopin is expressed postsynaptically in both synaptic layers of the retina. Functional disruption via morpholino antisense injection leads to characteristic defects in the electroretinogram and defects in visual contrast sensitivity. We therefore demonstrated that nyctalopin plays a similar role in retinal synapse function in the cone pathway as in the rod pathway, thereby creating a genetic model for CSNB1 and its effects on cone vision.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > Institute of Medical Molecular Genetics|
|DDC:||570 Life sciences; biology|
610 Medicine & health
|Date:||01 September 2006|
|Deposited On:||11 Feb 2008 13:11|
|Last Modified:||27 Nov 2013 23:59|
|Citations:||Web of Science®. Times cited: 10|
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