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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-4246

Raynaud, C; Papavinasasundaram, K G; Speight, R A; Springer, B; Sander, P; Böttger, E C; Colston, M J; Draper, P (2002). The functions of OmpATb, a pore-forming protein of Mycobacterium tuberculosis. Molecular Microbiology, 46(1):191-201.

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Abstract

The functions of OmpATb, the product of the ompATb gene of Mycobacterium tuberculosis and a putative porin, were investigated by studying a mutant with a targeted deletion of the gene, and by observing expression of the gene in wild-type M. tuberculosis H37Rv by real-time polymerase chain reaction (PCR) and immunoblotting. The loss of ompATb had no effect on growth under normal conditions, but caused a major reduction in ability to grow at reduced pH. The gene was substantially upregulated in wild-type bacteria exposed to these conditions. The mutant was impaired in its ability to grow in macrophages and in normal mice, although it was as virulent as the wild type in mice that lack T cells. Deletion of the ompATb gene reduced permeability to several small water-soluble substances. This was particularly evident at pH 5.5; at this pH, uptake of serine was minimal, suggesting that, at this pH, OmpATb might be the only functioning porin. These data indicate that OmpATb has two functions: as a pore-forming protein with properties of a porin, and in enabling M. tuberculosis to respond to reduced environmental pH. It is not known whether this second function is related to the porin-like activity at low pH or involves a completely separate role for OmpATB. The involvement with pH is likely to contribute to the ability of M. tuberculosis to overcome host defence mechanisms and grow in a mammalian host.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Medical Microbiology
DDC:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2002
Deposited On:26 Mar 2009 13:28
Last Modified:11 Dec 2013 15:11
Publisher:Wiley-Blackwell
ISSN:0950-382X
Publisher DOI:10.1046/j.1365-2958.2002.03152.x
PubMed ID:12366842
Citations:Web of Science®. Times Cited: 52
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Scopus®. Citation Count: 53

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