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DNA alkylation damage as a sensor of nitrosative stress in Mycobacterium tuberculosis


Durbach, S I; Springer, B; Machowski, E E; North, R J; Papavinasasundaram, K G; Colston, M J; Böttger, E C; Mizrahi, V (2003). DNA alkylation damage as a sensor of nitrosative stress in Mycobacterium tuberculosis. Infection and Immunity, 71(2):997-1000.

Abstract

One of the cellular consequences of nitrosative stress is alkylation damage to DNA. To assess whether nitrosative stress is registered on the genome of Mycobacterium tuberculosis, mutants lacking an alkylation damage repair and reversal operon were constructed. Although hypersensitive to the genotoxic effects of N-methyl-N'-nitro-N-nitrosoguanidine in vitro, the mutants displayed no phenotype in vivo, suggesting that permeation of nitrosative stress to the level of cytotoxic DNA damage is restricted.

One of the cellular consequences of nitrosative stress is alkylation damage to DNA. To assess whether nitrosative stress is registered on the genome of Mycobacterium tuberculosis, mutants lacking an alkylation damage repair and reversal operon were constructed. Although hypersensitive to the genotoxic effects of N-methyl-N'-nitro-N-nitrosoguanidine in vitro, the mutants displayed no phenotype in vivo, suggesting that permeation of nitrosative stress to the level of cytotoxic DNA damage is restricted.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Medical Microbiology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2003
Deposited On:26 Mar 2009 09:19
Last Modified:05 Apr 2016 12:29
Publisher:American Society for Microbiology
ISSN:0019-9567
Additional Information:Copyright: American Society for Microbiology
Publisher DOI:10.1128/IAI.71.2.997–1000.2003
PubMed ID:12540583
Permanent URL: http://doi.org/10.5167/uzh-4250

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