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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-4251

Wilson, R W; Steingrube, V A; Böttger, E C; Springer, B; Brown-Elliott, B A; Vincent, V; Jost, K C; Zhang, Y; Garcia, M J; Chiu, S H; Onyi, G O; Rossmoore, H; Nash, D R; Wallace, R J (2001). Mycobacterium immunogenum sp. nov., a novel species related to mycobacterium abscessus and associated with clinical disease, pseudo-outbreaks and contaminated metalworking fluids: an international cooperative study on mycobacterial taxonomy. International Journal of Systematic and Evolutionary Microbiology, 51(Pt 5):1751-1764.

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Abstract

PCR-restriction enzyme pattern analysis of a 439 bp hsp65 gene segment identified 113 unique isolates among non-pigmented rapidly growing mycobacteria (RGM) from clinical and environmental sources that failed to match currently recognized species patterns. This group represented 40% of isolates recovered from bronchoscope contamination pseudo-outbreaks, 0% of disease-associated nosocomial outbreaks and 4% of routine clinical isolates of the Mycobacterium abscessus/Mycobacterium chelonae group submitted to the Mycobacteria/Nocardia laboratory for identification. It is grouped within the Mycobacterium fortuitum complex, with growth in less than 7 d, absence of pigmentation, positive 3-d arylsulfatase reaction and growth on MacConkey agar without crystal violet. It exhibited overlapping biochemical, antimicrobial susceptibility and HPLC characteristics of M. abscessus and M. chelonae. By 16S rRNA gene sequencing, these isolates comprised a homogeneous group with a unique hypervariable region A sequence and differed by 8 and 10 bp, respectively, from M. abscessus and M. chelonae. Surprisingly, this taxon contained two copies of the ribosomal operon, compared with single copies in the two related species. By DNA-DNA hybridization, this new group exhibited <30% homology with recognized RGM species. The name Mycobacterium immunogenum sp. nov. is proposed for this new taxon.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Medical Microbiology
DDC:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2001
Deposited On:27 Mar 2009 07:41
Last Modified:27 Nov 2013 17:09
Publisher:Society for General Microbiology
ISSN:1466-5026
Publisher DOI:10.1099/00207713-51-5-1751
Official URL:http://ijs.sgmjournals.org/cgi/content/abstract/51/5/1751
PubMed ID:11594606
Citations:Web of Science®. Times Cited: 101
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Scopus®. Citation Count: 111

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