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Computer-based analysis of the optokinetic response in zebrafish larvae


Hodel, C; Neuhauss, S C F (2008). Computer-based analysis of the optokinetic response in zebrafish larvae. Cold Spring Harbor Protocols:4961.

Abstract

Large movements in the visual field trigger stereotypic eye movements in vertebrates. This optokinetic response
(OKR) is robust in zebrafish (Danio rerio) larvae older than 4 days post-fertilization (dpf), and is hence ideally
suited for use in the evaluation of visual performance after genetic manipulations. This protocol describes a simple
method by which a computer-generated visual stimulus is projected onto a screen and watched by immobilized
larvae. The resulting eye movements are recorded and evaluated automatically, using a digital video camera and
appropriate computer software. Depending on the sophistication of the software used, the evaluation can be
performed in real time during the recording session, or by analyzing the recorded movie files off-line.

Large movements in the visual field trigger stereotypic eye movements in vertebrates. This optokinetic response
(OKR) is robust in zebrafish (Danio rerio) larvae older than 4 days post-fertilization (dpf), and is hence ideally
suited for use in the evaluation of visual performance after genetic manipulations. This protocol describes a simple
method by which a computer-generated visual stimulus is projected onto a screen and watched by immobilized
larvae. The resulting eye movements are recorded and evaluated automatically, using a digital video camera and
appropriate computer software. Depending on the sophistication of the software used, the evaluation can be
performed in real time during the recording session, or by analyzing the recorded movie files off-line.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Molecular Life Sciences
Dewey Decimal Classification:570 Life sciences; biology
Uncontrolled Keywords:zebrafish, optokinetic response, visual behavior
Language:English
Date:2008
Deposited On:20 Jan 2009 12:00
Last Modified:05 Apr 2016 12:29
Publisher:Cold Spring Harbor Press
ISSN:1559-6095
Publisher DOI:10.1101/pdb.prot4961
Official URL:http://cshprotocols.cshlp.org/cgi/content/full/2008/4/pdb.prot4961
Permanent URL: http://doi.org/10.5167/uzh-4254

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