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Electroretinogram (ERG) measurements in larval zebrafish


Fleisch, V C; Jametti, T; Neuhauss, S C F (2008). Electroretinogram (ERG) measurements in larval zebrafish. Cold Spring Harbor Protocols:4973.

Abstract

The electroretinogram (ERG) is an electrophysiological tool used to measure electrical activity originating in the
outer retina in response to a light stimulus. Defects occurring at various levels of the retina can easily be
detected by ERG measurements. Furthermore, the shape of the ERG response points toward the likely retinal cell
type responsible for the deficit. Thus, this method is particularly useful for a rapid assessment of retinal function in genetically or pharmacologically manipulated animals. A typical ERG curve can be subdivided into three
components: a small initial a-wave originating in photoreceptor activity, a large positive b-wave reflecting mainly
ON bipolar cell depolarization, and a d-wave occurring at light offset. Here we present a noninvasive protocol for
taking ERG measurements in larval zebrafish (4-7 days post-fertilization [dpf]). We use an extracellular
recording electrode which is placed onto the surface of the cornea of the larva, and a light flash of a defined
intensity and duration which is applied to evoke a response. In a typical larval ERG trace, we are able to record
ERG a-, b-, and d-waves.

The electroretinogram (ERG) is an electrophysiological tool used to measure electrical activity originating in the
outer retina in response to a light stimulus. Defects occurring at various levels of the retina can easily be
detected by ERG measurements. Furthermore, the shape of the ERG response points toward the likely retinal cell
type responsible for the deficit. Thus, this method is particularly useful for a rapid assessment of retinal function in genetically or pharmacologically manipulated animals. A typical ERG curve can be subdivided into three
components: a small initial a-wave originating in photoreceptor activity, a large positive b-wave reflecting mainly
ON bipolar cell depolarization, and a d-wave occurring at light offset. Here we present a noninvasive protocol for
taking ERG measurements in larval zebrafish (4-7 days post-fertilization [dpf]). We use an extracellular
recording electrode which is placed onto the surface of the cornea of the larva, and a light flash of a defined
intensity and duration which is applied to evoke a response. In a typical larval ERG trace, we are able to record
ERG a-, b-, and d-waves.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Molecular Life Sciences
Dewey Decimal Classification:570 Life sciences; biology
Uncontrolled Keywords:zebrafish, electroretinogram, retina, photoreceptor
Language:English
Date:2008
Deposited On:20 Jan 2009 11:57
Last Modified:05 Apr 2016 12:29
Publisher:Cold Spring Harbor Laboratory Press
ISSN:1559-6095
Publisher DOI:10.1101/pdb.prot4973
Official URL:http://cshprotocols.cshlp.org/cgi/content/full/2008/4/pdb.prot4973
Permanent URL: http://doi.org/10.5167/uzh-4255

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