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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-42633

Hapfelmeier, S; Lawson, M A E; Slack, E; Kirundi, J K; Stoel, M; Heikenwalder, M; Cahenzli, J; Velykoredko, Y; Balmer, M L; Endt, K; Geuking, M B; Curtiss, R; McCoy, K D; Macpherson, A J (2010). Reversible microbial colonization of germ-free mice reveals the dynamics of IgA immune responses. Science, 328(5986):1705-1709.

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Abstract

The lower intestine of adult mammals is densely colonized with nonpathogenic (commensal) microbes. Gut bacteria induce protective immune responses, which ensure host-microbial mutualism. The continuous presence of commensal intestinal bacteria has made it difficult to study mucosal immune dynamics. Here, we report a reversible germ-free colonization system in mice that is independent of diet or antibiotic manipulation. A slow (more than 14 days) onset of a long-lived (half-life over 16 weeks), highly specific anticommensal immunoglobulin A (IgA) response in germ-free mice was observed. Ongoing commensal exposure in colonized mice rapidly abrogated this response. Sequential doses lacked a classical prime-boost effect seen in systemic vaccination, but specific IgA induction occurred as a stepwise response to current bacterial exposure, such that the antibody repertoire matched the existing commensal content.

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137 citations in Web of Science®
150 citations in Scopus®
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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Institute of Neuropathology
DDC:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2010
Deposited On:19 Jan 2011 17:41
Last Modified:27 Nov 2013 22:38
Publisher:American Association for the Advancement of Science (AAAS)
ISSN:0036-8075
Additional Information:Comment in: Science. 2010 Jun 25;328(5986):1646-7.
Publisher DOI:10.1126/science.1188454
PubMed ID:20576892

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