Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-42633
Hapfelmeier, S; Lawson, M A E; Slack, E; Kirundi, J K; Stoel, M; Heikenwalder, M; Cahenzli, J; Velykoredko, Y; Balmer, M L; Endt, K; Geuking, M B; Curtiss, R; McCoy, K D; Macpherson, A J (2010). Reversible microbial colonization of germ-free mice reveals the dynamics of IgA immune responses. Science, 328(5986):1705-1709.
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The lower intestine of adult mammals is densely colonized with nonpathogenic (commensal) microbes. Gut bacteria induce protective immune responses, which ensure host-microbial mutualism. The continuous presence of commensal intestinal bacteria has made it difficult to study mucosal immune dynamics. Here, we report a reversible germ-free colonization system in mice that is independent of diet or antibiotic manipulation. A slow (more than 14 days) onset of a long-lived (half-life over 16 weeks), highly specific anticommensal immunoglobulin A (IgA) response in germ-free mice was observed. Ongoing commensal exposure in colonized mice rapidly abrogated this response. Sequential doses lacked a classical prime-boost effect seen in systemic vaccination, but specific IgA induction occurred as a stepwise response to current bacterial exposure, such that the antibody repertoire matched the existing commensal content.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > University Hospital Zurich > Institute of Neuropathology|
|DDC:||570 Life sciences; biology|
610 Medicine & health
|Deposited On:||19 Jan 2011 17:41|
|Last Modified:||27 Nov 2013 22:38|
|Publisher:||American Association for the Advancement of Science (AAAS)|
|Additional Information:||Comment in: Science. 2010 Jun 25;328(5986):1646-7.|
|Citations:||Web of Science®. Times Cited: 134|
Scopus®. Citation Count: 148
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