Quick Search:

uzh logo
Browse by:

Zurich Open Repository and Archive

Maintenance: Tuesday, July the 26th 2016, 07:00-10:00

ZORA's new graphical user interface will be relaunched (For further infos watch out slideshow ZORA: Neues Look & Feel). There will be short interrupts on ZORA Service between 07:00am and 10:00 am. Please be patient.

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-4314

Blau, N; Thöny, B (2008). Pterins and related enzymes. In: Blau, N; Duran, M; Gibson, K M. Laboratory guide to the methods in biochemical genetics. Berlin Heidelberg, Germany: Springer Verlag, 665-701.

[img] PDF - Registered users only
View at publisher


Tetrahydrobiopterin(BH4)cofactor is essential for various enzyme activities, including phenylalanine-4-hydroxylase(PAH), tyrosine-3-hydroxylase(TH), tryptophan-5-hydroxylase(TPH), nitric oxide synthase(NOS), and glyceryl-ether monooxygen-ase(GEMO).The de novo biosynthesis pathway of BH4 from guanosine triphosphate (GTP) involves GTP cyclohydrolase I (GTPCH), 6-pyruvoyl-tetrahydropterin synthase (PTPS), and sepiapterin reductase (SR). Three additional enzymes catalyze the last two steps of reduction: aldose reductase (AR), carbonyl reductase (CR), and 3α-hydroxysteroid dehydrogenase type 2 (HSDH2). Cofactor regeneration requires pterin-4a-carbinolamine dehydratase(PCD)and dihydropteridine reductase (DHPR)[1].
The pteridines, a group to which BH4 also belongs, constitute naturally occurring compounds with a base structure of pyrazino [2,3d] pyrimidine (Fig. 6.1.1). Pteridines with the structure 2-amino-4-oxo are designated by the term “pterins,” and those with the structure 2,4-dioxo by the term“lumazines”(Fig.6.1.1). Two groups of pterins can be distinguished: the first have p-aminobenzoate and glutamate attached to the pterin.These pteridines are designated as conjugated pterins(e.g., folic acid). The second group consists of unconjugated pterins, which contain neither of these two substitutions; instead, substitutions occur at the 6-position of the pterin ring nucleus by the aliphatic side chain.The most important blue-fluorescing unconjugated pterins are neopterin, monapterin, biopterin, isoxanthopterin, primapterin, and pterin(Fig.6.1.1). Several other pterins,like xanthopterin, sepiapterin, and 3’-hydroxy-sepiepterin, are yellow-fluorescing species(Fig.6.1.2). Pterins can exist in different oxidation stages, but only fully reduced forms are biologically active.Biop-terin is present in biologicalsamples as biopterin, 7,8-dihydrobiopterin(BH2), and 5,6,7,8-tetrahydrobiopterin(BH4)(Fig.6.1.3).
In man, BH4 is degraded either nonenzymatically by side-chain cleavage to pterin or is enzymatically metabolized in the gastrointestinal tract to become a lumazine [2]. Pterin and dihydropterin are converted by xanthine dehydrogenase to isoxanthopterin and xanthopterin, respectively[3,4]. It is assumed, however, that most of the ingested BH4 is used as a cofactor(mainly for PAH in the liver)and is catabolized to nonfluorescing compounds;it may even be degraded to CO2 and ammonia.




4 downloads since deposited on 05 Dec 2008
0 downloads since 12 months

Detailed statistics

Additional indexing

Item Type:Book Section, refereed, further contribution
Communities & Collections:04 Faculty of Medicine > University Children's Hospital Zurich > Medical Clinic
Dewey Decimal Classification:610 Medicine & health
Date:31 May 2008
Deposited On:05 Dec 2008 10:51
Last Modified:05 Apr 2016 12:30
Publisher:Springer Verlag
ISBN:978-3-540-76697-1 (Print) 978-3-540-76698-8 (Online)
Publisher DOI:10.1007/978-3-540-76698-8_28
Related URLs:http://www.springerlink.com

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page