Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-43341
Moretti, M; Grunau, A; Minerdi, D; Gehrig, P; Roschitzki, B; Eberl, L; Garibaldi, A; Gullino, M L; Riedel, K (2010). A proteomics approach to study synergistic and antagonistic interactions of the fungal-bacterial consortium Fusarium oxysporum wild-type MSA 35. Proteomics, 10(18):3292-3320.
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Fusarium oxysporum is an important plant pathogen that causes severe damage of many economically important crop species. Various microorganisms have been shown to inhibit this soil-borne plant pathogen, including non-pathogenic F. oxysporum strains. In this study, F. oxysporum wild-type (WT) MSA 35, a biocontrol multispecies consortium that consists of a fungus and numerous rhizobacteria mainly belonging to gamma-proteobacteria, was analyzed by two complementary metaproteomic approaches (2-DE combined with MALDI-Tof/Tof MS and 1-D PAGE combined with LC-ESI-MS/MS) to identify fungal or bacterial factors potentially involved in antagonistic or synergistic interactions between the consortium members. Moreover, the proteome profiles of F. oxysporum WT MSA 35 and its cured counter-part CU MSA 35 (WT treated with antibiotics) were compared with unravel the bacterial impact on consortium functioning. Our study presents the first proteome mapping of an antagonistic F. oxysporum strain and proposes candidate proteins that might play an important role for the biocontrol activity and the close interrelationship between the fungus and its bacterial partners.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > Functional Genomics Center Zurich|
07 Faculty of Science > Institute of Plant Biology
|DDC:||570 Life sciences; biology|
580 Plants (Botany)
610 Medicine & health
|Deposited On:||27 Jan 2011 19:30|
|Last Modified:||27 Nov 2013 19:45|
|Citations:||Web of Science®. Times cited: 5|
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