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Weder, G; Blondiaux, N; Giazzon, M; Matthey, N; Klein, M; Pugin, R; Heinzelmann, H; Liley, M (2010). Use of force spectroscopy to investigate the adhesion of living adherent cells. Langmuir, 26(11):8180-8186.

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Abstract

The use of force spectroscopy to study the adhesion of living fibroblasts to their culture substrate was investigated. Both primary fibroblasts (PEMF) and a continuous cell line (3T3) were studied on quartz surfaces. Using a fibronectin-coated AFM cantilever, it was possible to detach a large proportion of the 3T3 cells from the quartz surfaces. Their adhesion to the quartz surface and the effects of topography on this adhesion could be quantified. Three parameters characteristic of the adhesion were measured: the maximum force of detachment, the work of adhesion, and the distance of detachment. Few PEMF cells were detached under the same experimental conditions. The potential and limitations of this method in measuring cell/surface interactions for adherent cells are discussed.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Biomedical Engineering
DDC:170 Ethics
610 Medicine & health
Language:English
Date:2010
Deposited On:26 Jan 2011 14:40
Last Modified:28 Nov 2013 01:52
Publisher:American Chemical Society
ISSN:0743-7463
Publisher DOI:10.1021/la904526u
Related URLs:http://pubs.acs.org/doi/abs/10.1021/la904526u (Publisher)
PubMed ID:20146500
Citations:Web of Science®. Times Cited: 9
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