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Amino acid analysis by precolumn derivatization with 1-fluoro-2,4-dinitrophenyl-5-L-alanine amide (Marfey agent)


Kochhar, S; Mouratou, B; Christen, P (2009). Amino acid analysis by precolumn derivatization with 1-fluoro-2,4-dinitrophenyl-5-L-alanine amide (Marfey agent). In: Walker, J M. The Protein Protocols Handbook. Totowa, NJ: Humana Press, 567-572.

Abstract

For the determination of the chirality of amino acids and other compounds containing primary amino groups, derivatization with the chiral Marfey’s reagent has kept its place as a complementary method beside direct chromatographic analysis with chiral stationary phases. Here, we describe the use of Marfey’s reagent for common amino acid analysis. The simplicity of pre-column derivatization of amino acids with this reagent, the ease of separation of the derivatized amino acids with non-dedicated reverse-phase highperformance liquid chromatography (RP-HPLC) equipment and the high sensitivity of their chromatographic detection at 340 nm make the described method an attractive alternative to the dedicated amino acid analyzer.

For the determination of the chirality of amino acids and other compounds containing primary amino groups, derivatization with the chiral Marfey’s reagent has kept its place as a complementary method beside direct chromatographic analysis with chiral stationary phases. Here, we describe the use of Marfey’s reagent for common amino acid analysis. The simplicity of pre-column derivatization of amino acids with this reagent, the ease of separation of the derivatized amino acids with non-dedicated reverse-phase highperformance liquid chromatography (RP-HPLC) equipment and the high sensitivity of their chromatographic detection at 340 nm make the described method an attractive alternative to the dedicated amino acid analyzer.

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Additional indexing

Item Type:Book Section, refereed, further contribution
Communities & Collections:04 Faculty of Medicine > Department of Biochemistry
07 Faculty of Science > Department of Biochemistry
Dewey Decimal Classification:570 Life sciences; biology
Uncontrolled Keywords:Amino acid analysis; Marfey’s reagent; 1-fluoro-2,4-dinitrophenyl-5-Lalanina amide; pre-column derivatization; high-performance liquid chromatography; enantiomers; diastereomers.
Language:English
Date:1 March 2009
Deposited On:24 Oct 2008 11:35
Last Modified:14 Sep 2016 13:36
Publisher:Humana Press
Number:3rd ed
ISBN:978-1-58829-880-5
Official URL:http://www.springer.com/978-1-58829-880-5
Related URLs:http://www.recherche-portal.ch/primo_library/libweb/action/search.do?fn=search&mode=Advanced&vid=ZAD&vl%28186672378UI0%29=isbn&vl%281UI0%29=contains&vl%28freeText0%29=978-1-58829-880-5
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http://www.springer.com/978-1-58829-880-5
Permanent URL: http://doi.org/10.5167/uzh-4371

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