Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-4414
Bange, F-C; Böttger, E C (2002). Improved decontamination method for recovering mycobacteria from patients with cystic fibrosis. European Journal of Clinical Microbiology and Infectious Diseases, 21(7):546-548.
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In order to improve the recovery of mycobacteria from patients with cystic fibrosis, the present study evaluated a two-step decontamination procedure for clinical specimens. A total of 920 specimens obtained from 239 patients with cystic fibrosis were treated initially with N-acetyl-L-cysteine/sodium hydroxide. Of these specimens, 31 (3.3%) showed mycobacterial growth and 415 (45.1%) remained contaminated. Contaminated specimens were then subjected to a second round of decontamination, using a combination of N-acetyl-L-cysteine/sodium hydroxide and oxalic acid. Following this second decontamination, the number of specimens overgrown by microorganisms other than mycobacteria was reduced to 7.3%, and an additional 10 specimens positive for mycobacteria were found. The results suggest this two-step protocol could improve the recovery of mycobacteria from heavily contaminated specimens.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > Institute of Medical Microbiology|
|DDC:||570 Life sciences; biology|
610 Medicine & health
|Deposited On:||26 Mar 2009 13:48|
|Last Modified:||01 Dec 2013 02:51|
|Citations:||Web of Science®. Times cited: 6|
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