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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-4414

Bange, F-C; Böttger, E C (2002). Improved decontamination method for recovering mycobacteria from patients with cystic fibrosis. European Journal of Clinical Microbiology and Infectious Diseases, 21(7):546-548.

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Abstract

In order to improve the recovery of mycobacteria from patients with cystic fibrosis, the present study evaluated a two-step decontamination procedure for clinical specimens. A total of 920 specimens obtained from 239 patients with cystic fibrosis were treated initially with N-acetyl-L-cysteine/sodium hydroxide. Of these specimens, 31 (3.3%) showed mycobacterial growth and 415 (45.1%) remained contaminated. Contaminated specimens were then subjected to a second round of decontamination, using a combination of N-acetyl-L-cysteine/sodium hydroxide and oxalic acid. Following this second decontamination, the number of specimens overgrown by microorganisms other than mycobacteria was reduced to 7.3%, and an additional 10 specimens positive for mycobacteria were found. The results suggest this two-step protocol could improve the recovery of mycobacteria from heavily contaminated specimens.

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7 citations in Web of Science®
9 citations in Scopus®
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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Medical Microbiology
DDC:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2002
Deposited On:26 Mar 2009 12:48
Last Modified:01 Dec 2013 01:51
Publisher:Springer
ISSN:0934-9723
Publisher DOI:10.1007/s10096-002-0760-y
PubMed ID:12172748

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