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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-44883

Guetg, C; Lienemann, P; Sirri, V; Grummt, I; Hernandez-Verdun, D; Hottiger, M O; Fussenegger, M; Santoro, R (2010). The NoRC complex mediates the heterochromatin formation and stability of silent rRNA genes and centromeric repeats. The EMBO Journal, 29(13):2135-2146.

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Abstract

Maintenance of specific heterochromatic domains is crucial for genome stability. In eukaryotic cells, a fraction of the tandem-repeated ribosomal RNA (rRNA) genes is organized in the heterochromatic structures. The principal determinant of rDNA silencing is the nucleolar remodelling complex, NoRC, that consists of TIP5 (TTF-1-interacting protein-5) and the ATPase SNF2h. Here we showed that TIP5 not only mediates the establishment of rDNA silencing but also the formation of perinucleolar heterochromatin that contains centric and pericentric repeats. Our data indicated that the TIP5-mediated heterochromatin is indispensable for stability of silent rRNA genes and of major and minor satellite repeats. Moreover, depletion of TIP5 impairs rDNA silencing, upregulates rDNA transcription levels and induces cell transformation. These findings point to a role of TIP5 in protecting genome stability and suggest that it can play a role in the cellular transformation process.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Clinic for Obstetrics
05 Vetsuisse Faculty > Institute of Veterinary Biochemistry and Molecular Biology
DDC:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2010
Deposited On:22 Feb 2011 20:10
Last Modified:30 Nov 2013 15:56
Publisher:Nature Publishing Group
ISSN:0261-4189
Additional Information:Erratum in: EMBO J. 2010 Jul 7;29(13):2253.
Free access at:PubMed ID. An embargo period may apply.
Publisher DOI:10.1038/emboj.2010.17
PubMed ID:20168299

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