Quick Search:

uzh logo
Browse by:

Zurich Open Repository and Archive

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-44883

Guetg, C; Lienemann, P; Sirri, V; Grummt, I; Hernandez-Verdun, D; Hottiger, M O; Fussenegger, M; Santoro, R (2010). The NoRC complex mediates the heterochromatin formation and stability of silent rRNA genes and centromeric repeats. The EMBO Journal, 29(13):2135-2146.

[img] PDF (Verlags-PDF) - Registered users only
View at publisher


Maintenance of specific heterochromatic domains is crucial for genome stability. In eukaryotic cells, a fraction of the tandem-repeated ribosomal RNA (rRNA) genes is organized in the heterochromatic structures. The principal determinant of rDNA silencing is the nucleolar remodelling complex, NoRC, that consists of TIP5 (TTF-1-interacting protein-5) and the ATPase SNF2h. Here we showed that TIP5 not only mediates the establishment of rDNA silencing but also the formation of perinucleolar heterochromatin that contains centric and pericentric repeats. Our data indicated that the TIP5-mediated heterochromatin is indispensable for stability of silent rRNA genes and of major and minor satellite repeats. Moreover, depletion of TIP5 impairs rDNA silencing, upregulates rDNA transcription levels and induces cell transformation. These findings point to a role of TIP5 in protecting genome stability and suggest that it can play a role in the cellular transformation process.


57 citations in Web of Science®
52 citations in Scopus®
Google Scholar™



0 downloads since deposited on 22 Feb 2011
0 downloads since 12 months

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Clinic for Obstetrics
05 Vetsuisse Faculty > Institute of Veterinary Biochemistry and Molecular Biology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Deposited On:22 Feb 2011 20:10
Last Modified:17 Jun 2016 09:28
Publisher:Nature Publishing Group
Additional Information:Erratum in: EMBO J. 2010 Jul 7;29(13):2253.
Free access at:PubMed ID. An embargo period may apply.
Publisher DOI:10.1038/emboj.2010.17
PubMed ID:20168299

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page