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Santangelo, M G; Antoni, P M; Spingler, B; Jeschke, G (2010). Can copper(II) mediate Hoogsteen base-pairing in a left-handed DNA duplex? A pulse EPR study. ChemPhysChem, 11(3):599-606.

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Abstract

Pulse EPR spectroscopy is sued to investigate possible structural features of the copper(II) ion coordinated to poly(dG-dC) poly(dG-dC) in a frozen aqueous solution, and the structural change of the polynucleotide induced by the presence of the metal ion. Two different copper species were identified and their geometry explained by a molecular model. According to this model, one species is exclusively coordinated to a single guanine with the N7 nitrogen atom forming a coordinative bond with the copper. In the other species, a guanine and a cytosine form a ternary complex together with the copper ion. A copper crosslink between the N7 of guanine and N3 of cytosine is proposed as the most probable coordination site. Moreover, no evidence was found for an interaction of either copper species with a phosphate group or equatorial water molecules. In addition, circular dichroism (CD) spectroscopy showed that the DNA of the Cu-II-poly(dG-dC)center dot poly(dG-dC) adducts resembles the left-handed Z-form. These results suggest that metal-mediated Hoogsteen base pairing, as previously proposed for a right-handed DNA duplex, can also occur in a double-stranded left-handed DNA.

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Chemistry
DDC:540 Chemistry
Uncontrolled Keywords:copper; DNA structures; nucleobases; pulse EPIR spectroscopy; structure elucidation
Language:English
Date:2010
Deposited On:08 Feb 2011 13:34
Last Modified:27 Nov 2013 17:35
Publisher:Wiley-Blackwell
ISSN:1439-4235
Funders:ETH Zurich, University of Zurich
Publisher DOI:10.1002/cphc.200900672
PubMed ID:20029882
Other Identification Number:ISI:000275243700010
Citations:Web of Science®. Times Cited: 8
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Scopus®. Citation Count: 9

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