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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-4532

Yue, T; et al; Blumenthal, S (2008). Postconditioning with a volatile anaesthetic in alveolar epithelial cells in vitro. European Respiratory Journal, 31(1):118-125.

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Acute lung injury is a common complication in critically ill patients. The present study examined possible immunomodulating effects of the volatile anaesthetic sevoflurane on lipopolysaccharide (LPS)-stimulated alveolar epithelial cells (AEC) in vitro. Sevoflurane was applied after the onset of injury, simulating a "postconditioning" scenario. Rat AEC were stimulated with LPS for 2 h, followed by a 4-h co-exposure to a CO(2)/air mixture with sevoflurane 2.2 volume %; control cells were exposed to the CO(2)/air mixture only. Cytokine-induced neutrophil chemoattractant-1, monocyte chemoattractant protein-1, intercellular adhesion molecule-1, as well as the potential protective mediators inducible nitric oxide synthase (iNOS)2 and heat shock protein (HSP)-32, were analysed. Additionally, functional assays (chemotaxis, adherence and cytotoxicity assay) were performed. A significant reduction of inflammatory mediators in LPS-stimulated, sevoflurane-exposed AEC was found, leading to reduced chemotaxis, neutrophil adherence and neutrophil-induced AEC killing. While iNOS2 was increased in the sevoflurane group, blocking experiments with iNOS2 inhibitor did not affect sevoflurane-induced decrease of inflammatory mediators and AEC killing. Interestingly, sevoflurane treatment also resulted in an enhanced expression of HSP-32. The data presented in the current study provide strong evidence that anaesthetic postconditioning with sevoflurane mediates cytoprotection in the respiratory compartment in an in vitro model of acute lung injury.


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Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Center for Integrative Human Physiology
04 Faculty of Medicine > University Hospital Zurich > Institute of Anesthesiology
04 Faculty of Medicine > Institute of Physiology
07 Faculty of Science > Institute of Physiology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Date:January 2008
Deposited On:24 Oct 2008 09:08
Last Modified:05 Apr 2016 12:30
Publisher:European Respiratory Society
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:10.1183/09031936.00046307
Official URL:http://erj.ersjournals.com/cgi/content/full/31/1/118?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&author1=yue&searchid=1&FIRSTINDEX=0&sortspec=relevance&volume=31&resourcetype=HWCIT
PubMed ID:17898018

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