Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-45467
Keinath, N F; Kierszniowska, S; Lorek, J; Bourdais, G; Kessler, S A; Shimosato-Asano, H; Grossniklaus, U; Schulze, W X; Robatzek, S; Panstruga, R (2010). PAMP (pathogen-associated molecular pattern)-induced changes in plasma membrane compartmentalization reveal novel components of plant immunity. The Journal of Biological Chemistry, 285(50):39140-39149.
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Plasma membrane compartmentalization spatiotemporally regulates cell-autonomous immune signaling in animal cells. To elucidate immediate early protein dynamics at the plant plasma membrane in response to the bacterial pathogen-associated molecular pattern (PAMP) flagellin (flg22) we employed quantitative mass spectrometric analysis on detergent-resistant membranes (DRMs) of Arabidopsis thaliana suspension cells. This approach revealed rapid and profound changes in DRM protein composition following PAMP treatment, prominently affecting proton ATPases and receptor-like kinases, including the flagellin receptor FLS2. We employed reverse genetics to address a potential contribution of a subset of these proteins in flg22-triggered cellular responses. Mutants of three candidates (DET3, AHA1, FER) exhibited a conspicuous defect in the PAMP-triggered accumulation of reactive oxygen species. In addition, these mutants showed altered mitogen-activated protein kinase (MAPK) activation, a defect in PAMP-triggered stomatal closure as well as altered bacterial infection phenotypes, which revealed three novel players in elicitor-dependent oxidative burst control and innate immunity. Our data provide evidence for dynamic elicitor-induced changes in the membrane compartmentalization of PAMP signaling components.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||07 Faculty of Science > Institute of Plant Biology|
07 Faculty of Science > Zurich-Basel Plant Science Center
|DDC:||580 Plants (Botany)|
|Deposited On:||11 Feb 2011 16:01|
|Last Modified:||10 Mar 2014 14:51|
|Publisher:||American Society for Biochemistry and Molecular Biology|
|Additional Information:||This research was originally published in: Keinath, N F; Kierszniowska, S; Lorek, J; Bourdais, G; Kessler, S A; Shimosato-Asano, H; Grossniklaus, U; Schulze, W X; Robatzek, S; Panstruga, R (2010). PAMP (pathogen-associated molecular pattern)-induced changes in plasma membrane compartmentalization reveal novel components of plant immunity. The Journal of Biological Chemistry, 285(50):39140-39149. © the American Society for Biochemistry and Molecular Biology.|
|Citations:||Web of Science®. Times Cited: 54|
Scopus®. Citation Count: 62
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