Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-45598
Höög, J L; Huisman, S M; Sebö-Lemke, Z; Sandblad, L; McIntosh, J R; Antony, C; Brunner, D (2011). Electron tomography reveals a flared morphology on growing microtubule ends. Journal of Cell Science, 124(Pt 5):693-698.
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Microtubules (MTs) exhibit dynamic instability, alternating between phases of growth and shortening, mostly at their uncapped plus ends. Based on results from cryo-electron microscopy it was proposed that growing MTs display mainly curved sheets and blunt ends; during depolymerisation curled 'ramshorns' predominate. Observations of MTs in mitotic cells have suggested that the situation in vivo differs from that in vitro, but so far, a clear comparison between in vivo and in vitro results has not been possible because MT end structures could not be correlated directly with the dynamic state of that particular MT. Here we combine light microscopy and electron tomography (ET) to show that growing MT plus ends in the fission yeast Schizosaccharomyces pombe display predominantly a flared morphology. This indicates that MT polymerisation in vivo and in vitro can follow different paths.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||07 Faculty of Science > Institute of Molecular Life Sciences|
|DDC:||570 Life sciences; biology|
|Uncontrolled Keywords:||Microtubule polymerisation, Plus ends, Electron tomography, Schizosaccharomyces pombe|
|Date:||08 February 2011|
|Deposited On:||22 Feb 2011 15:40|
|Last Modified:||27 Nov 2013 20:33|
|Publisher:||Company of Biologists|
|Citations:||Web of Science®. Times Cited: 7|
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