Quick Search:

uzh logo
Browse by:

The deadline for the annual report 2015 is January 31st, 2016

Zurich Open Repository and Archive

Maintenance: Tuesday, 16.2.2015, 06:00-08:00

Maintenance work on various system components of ZORA. During this time there will be a brief unavailability for about 1 hour. Please be patient.

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-45598

Höög, J L; Huisman, S M; Sebö-Lemke, Z; Sandblad, L; McIntosh, J R; Antony, C; Brunner, D (2011). Electron tomography reveals a flared morphology on growing microtubule ends. Journal of Cell Science, 124(Pt 5):693-698.

[img] PDF - Registered users only
View at publisher


Microtubules (MTs) exhibit dynamic instability, alternating between phases of growth and shortening, mostly at their uncapped plus ends. Based on results from cryo-electron microscopy it was proposed that growing MTs display mainly curved sheets and blunt ends; during depolymerisation curled 'ramshorns' predominate. Observations of MTs in mitotic cells have suggested that the situation in vivo differs from that in vitro, but so far, a clear comparison between in vivo and in vitro results has not been possible because MT end structures could not be correlated directly with the dynamic state of that particular MT. Here we combine light microscopy and electron tomography (ET) to show that growing MT plus ends in the fission yeast Schizosaccharomyces pombe display predominantly a flared morphology. This indicates that MT polymerisation in vivo and in vitro can follow different paths.


10 citations in Web of Science®
12 citations in Scopus®
Google Scholar™



2 downloads since deposited on 22 Feb 2011
0 downloads since 12 months

Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Molecular Life Sciences
Dewey Decimal Classification:570 Life sciences; biology
Uncontrolled Keywords:Microtubule polymerisation, Plus ends, Electron tomography, Schizosaccharomyces pombe
Date:8 February 2011
Deposited On:22 Feb 2011 14:40
Last Modified:27 Nov 2013 19:33
Publisher:Company of Biologists
Publisher DOI:10.1242/jcs.072967
PubMed ID:21303925

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page