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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-4575

Ackermann, G E; Domenighetti, A A; Deten, A; Bonath, I; Marenholz, I; Pedrazzini, T; Erne, P; Heizmann, C W (2008). S100A1 deficiency results in prolonged ventricular repolarization in response to sympathetic activation. General Physiology and Biophysics, 27(2):127-142.

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Abstract

S100A1 is a Ca(2+)-binding protein and predominantly expressed in the heart. We have generated a mouse line of S100A1 deficiency by gene trap mutagenesis to investigate the impact of S100A1 ablation on heart function. Electrocardiogram recordings revealed that after beta-adrenergic stimulation S100A1-deficient mice had prolonged QT, QTc and ST intervals and intraventricular conduction disturbances reminiscent of 2 : 1 bundle branch block. In order to identify genes affected by the loss of S100A1, we profiled the mutant and wild type cardiac transcriptomes by gene array analysis. The expression of several genes functioning to the electrical activity of the heart were found to be significantly altered. Although the default prediction would be that mRNA and protein levels are highly correlated, comprehensive immunoblot analyses of salient up- or down-regulated candidate genes of any cellular network revealed no significant changes on protein level. Taken together, we found that S100A1 deficiency results in cardiac repolarization delay and alternating ventricular conduction defects in response to sympathetic activation accompanied by a significantly different transcriptional regulation.

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Veterinary Physiology
04 Faculty of Medicine > University Children's Hospital Zurich > Medical Clinic
DDC:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2008
Deposited On:27 Oct 2008 08:40
Last Modified:27 Nov 2013 20:21
Publisher:Slovenska Akademia Vied
ISSN:0231-5882
Official URL:http://gpb.aepress.sk/casopisy/01AV.php?file=2008_02_127.pdf
PubMed ID:18645228
Citations:Web of Science®. Times Cited: 10
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