Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-4576
Buetler, T M; Leclerc, E; Baumeyer, A; Latado, H; Newell, J; Adolfsson, O; Parisod, V; Richoz, J; Maurer, S; Foata, F; Piguet, D; Junod, S; Heizmann, C W; Delatour, T (2008). N(epsilon)-carboxymethyllysine-modified proteins are unable to bind to RAGE and activate an inflammatory response. Molecular Nutrition and Food Research, 52(3):370-378.
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Advanced glycation endproducts (AGEs) containing carboxymethyllysine (CML) modifications are generally thought to be ligands of the receptor for AGEs, RAGEs. It has been argued that this results in the activation of pro-inflammatory pathways and diseases. However, it has not been shown conclusively that a CML-modified protein can interact directly with RAGE. Here, we have analyzed whether beta-lactoglobulin (bLG) or human serum albumin (HSA) modified chemically to contain only CML (10-40% lysine modification) can (i) interact with RAGE in vitro and (ii) interact with and activate RAGE in lung epithelial cells. Our results show that CML-modified bLG or HSA are unable to bind to RAGE in a cell-free assay system (Biacore). Furthermore, they are unable to activate pro-inflammatory signaling in the cellular system. Thus, CML probably does not form the necessary structure(s) to interact with RAGE and activate an inflammatory signaling cascade in RAGE-expressing cells.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > University Children's Hospital Zurich > Medical Clinic|
|DDC:||610 Medicine & health|
|Deposited On:||27 Oct 2008 10:43|
|Last Modified:||28 Nov 2013 02:21|
|Citations:||Web of Science®. Times cited: 30|
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