Kriz, A; Schmid, K; Baumgartner, N; Ziegler, U; Berger, I; Ballmer-Hofer, K; Berger, P (2010). A plasmid-based multigene expression system for mammalian cells. Nature Communications, 1(8):Article number: 120.
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The introduction of heterologous genetic information, particularly of multiple genes, into mammalian cells is a key technology in contemporary experimental biological research. The coexpression of fluorescently tagged sensors is required to simultaneously analyse multiple parameters in living cells and the coexpression of several proteins is necessary to manipulate cell fate in stem cell biology. Current technologies for multigene expression in mammalian cells are inefficient, inflexible and time-consuming. In this paper we describe MultiLabel, a novel and highly efficient modular plasmid-based eukaryotic expression system. Independent expression vectors are assembled by a Cre/LoxP reaction into a plasmid with multiple expression cassettes. MultiLabel enables rapid construction of multigene expression vectors for the single-step creation of transiently or stably transfected mammalian cells.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > Center for Microscopy and Image Analysis|
|DDC:||570 Life sciences; biology|
610 Medicine & health
|Deposited On:||22 Feb 2011 11:27|
|Last Modified:||28 Nov 2013 00:01|
|Publisher:||Nature Publishing Group|
|Citations:||Web of Science®. Times cited: 10|
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