Quick Search:

uzh logo
Browse by:

Zurich Open Repository and Archive

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-4642

Polymenidou, M; Trusheim, H; Stallmach, L; Moos, R; Julius, C; Miele, G; Lenz-Bauer, C; Aguzzi, A (2008). Canine MDCK cell lines are refractory to infection with human and mouse prions. Vaccine, 26(21):2601-2614.

Accepted Version
View at publisher


Influenza vaccine production in embryonated eggs is associated with many disadvantages, and production in cell culture systems is a viable alternative. Madin Darby canine kidney (MDCK) cells are permissive for a variety of orthomyxoviruses and have proven particularly suitable for vaccine mass production. However, mammalian cells harboring the Prnp gene can theoretically acquire prion infections. Here, we have attempted to infect MDCK cells and substrains thereof with prions. We found that MDCK cells did not produce any protease-resistant PrP(Sc) upon exposure to brain homogenates derived from humans suffering from Creutzfeldt-Jakob disease (CJD) or from mice infected with Rocky Mountain Laboratory (RML) scrapie prions. Further, transmission of MDCK lysates to N2aPK1 cells did not induce formation of PrP(Sc) in the latter. PrP(C) biogenesis and processing in MDCK cells were similar to those of prion-sensitive N2aPK1 cells. However, steady-state levels of PrP(C) were very low, and PrP(C) did not partition with detergent-resistant membranes upon density gradient analysis. These factors may account for their resistance to infection. Alternatively, prion resistance may be related to the specific sequence of canine Prnp, as suggested by the lack of documented prion diseases in dogs.


22 citations in Web of Science®
26 citations in Scopus®
Google Scholar™



126 downloads since deposited on 31 Oct 2008
19 downloads since 12 months

Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Institute of Neuropathology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Deposited On:31 Oct 2008 08:12
Last Modified:05 Apr 2016 12:31
Publisher DOI:10.1016/j.vaccine.2008.03.035
PubMed ID:18423803

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page