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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-46441

Blom, J F; Zimmermann, Y S; Ammann, T; Pernthaler, J (2010). Scent of danger: floc formation by a freshwater bacterium is induced by supernatants from a predator-prey coculture. Applied and Environmental Microbiology, 76(18):6156-6163.

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Abstract

We investigated predator-prey interactions in a model system consisting of the bacterivorous flagellate Poterioochromonas sp. strain DS and the freshwater bacterium Sphingobium sp. strain Z007. This bacterial strain tends to form a subpopulation of grazing-resistant microscopic flocs, presumably by aggregation. Enhanced formation of such flocs could be demonstrated in static batch culture experiments in the presence of the predator. The ratio of aggregates to single cells reached >0.1 after 120 h of incubation in an oligotrophic growth medium. The inoculation of bacteria into supernatants from cocultures of bacteria and flagellates (grown in oligotrophic or in rich media) also resulted in a substantially higher level of floc formation than that in supernatants from bacterial monocultures only. After separation of supernatants on a C(18) cartridge, the aggregate-inducing activity could be assigned to the 50% aqueous methanolic fraction, and further separation of this bioactive fraction could be achieved by high-pressure liquid chromatography. These results strongly suggest the involvement of one or several chemical factors in the induction of floc formation by Sphingobium sp. strain Z007 that are possibly released into the surrounding medium by flagellate grazing.

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Plant Biology
DDC:580 Plants (Botany)
Language:English
Date:2010
Deposited On:24 Feb 2011 17:30
Last Modified:23 Dec 2013 11:55
Publisher:American Society for Microbiology
ISSN:0099-2240
Publisher DOI:10.1128/AEM.01455-10
PubMed ID:20656874
Citations:Web of Science®. Times Cited: 3
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Scopus®. Citation Count: 3

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