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Characterization of bioactive cyclic oligopeptides of freshwater cyanobacteria (microcystins, cyanopeptolins, cyclamides)


Blom, Judith F; Höger, Stefan J; Jüttner, Friedrich (2010). Characterization of bioactive cyclic oligopeptides of freshwater cyanobacteria (microcystins, cyanopeptolins, cyclamides). In: Bagchi, S N; Kleiner, D; Mohanty, P. Protocols on algal and cyanobacterial research. New Delhi, India: Narosa Publishing, 53-70.

Abstract

Cyanobacteria produce a variety of unusual secondary metabolites, the function of some of them still remains unclear. Research in the last years has primarily focused on cyanobacterial microcystins that have severe impact on humans and livestock. While at the beginning the main focus point was on the structure elucidation and functionality of
microcystins, in recent years also cyanopeptolins and cyclamides have become important issues. Today also the possible ecological role of these metabolites is an attractive goal of research. In this protocol methods for the extraction, determination, and quantification using
high-performance liquid chromatography (HPLC), liquid chromatography-mass spectrometry (LC-MS), and gas chromatography-mass spectrometry (GC-MS) are described as well as methods (bioassay-guided fractionation, enzyme linked immunosorbent assay) to find toxins and protease inhibitors in cyanobacterial extracts. In addition, methods for the determination of the molar absorption coefficient, necessary for the quantification of small amounts of the
oligopeptides, are described.

Cyanobacteria produce a variety of unusual secondary metabolites, the function of some of them still remains unclear. Research in the last years has primarily focused on cyanobacterial microcystins that have severe impact on humans and livestock. While at the beginning the main focus point was on the structure elucidation and functionality of
microcystins, in recent years also cyanopeptolins and cyclamides have become important issues. Today also the possible ecological role of these metabolites is an attractive goal of research. In this protocol methods for the extraction, determination, and quantification using
high-performance liquid chromatography (HPLC), liquid chromatography-mass spectrometry (LC-MS), and gas chromatography-mass spectrometry (GC-MS) are described as well as methods (bioassay-guided fractionation, enzyme linked immunosorbent assay) to find toxins and protease inhibitors in cyanobacterial extracts. In addition, methods for the determination of the molar absorption coefficient, necessary for the quantification of small amounts of the
oligopeptides, are described.

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Additional indexing

Item Type:Book Section, refereed, further contribution
Communities & Collections:07 Faculty of Science > Department of Plant and Microbial Biology
Dewey Decimal Classification:580 Plants (Botany)
Language:English
Date:2010
Deposited On:24 Feb 2011 17:00
Last Modified:05 Apr 2016 14:49
Publisher:Narosa Publishing
ISBN:978-81-8487-059-6 (Narosa) ; 978-1-84265-584-9 (Alpha Science International)
Additional Information:Das Buch erschien auch: Oxford, U.K. : Alpha Science International, 2010. - 978-1-8426-5584-9
Related URLs:http://www.narosa.com/books_display.asp?catgcode=978-81-8487-059-6 (Publisher)
Permanent URL: http://doi.org/10.5167/uzh-46454

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