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Controlling ribozyme activity by metal ions


Schnabl, J; Sigel, Roland K O (2010). Controlling ribozyme activity by metal ions. Current Opinion in Chemical Biology, 14(2):269-275.

Abstract

The observed rates of ribozyme cleavage reactions are strongly dependent on the nature of the metal ion present Metal ions can thereby exhibit a stronger inhibiting or accelerating effect compared to Mg2+, which is usually considered the natural cofactor. Alkaline, alkaline earth, transition, d(10), and other metal ions are applied either to gain a spectroscopic handle on the metal center, and/or to elucidate the catalytic mechanism. Here we shortly review some of the most recent publications on the influence of different metal ions on catalysis of the hammerhead, hepatitis delta virus, and group II intron ribozymes. Comparison of the observed cleavage rates of hammerhead nbozymes with the metal ion affinities of different ligands reveals that these rates correlate perfectly with the intrinsic phosphate affinities of the metal ions involved.

Abstract

The observed rates of ribozyme cleavage reactions are strongly dependent on the nature of the metal ion present Metal ions can thereby exhibit a stronger inhibiting or accelerating effect compared to Mg2+, which is usually considered the natural cofactor. Alkaline, alkaline earth, transition, d(10), and other metal ions are applied either to gain a spectroscopic handle on the metal center, and/or to elucidate the catalytic mechanism. Here we shortly review some of the most recent publications on the influence of different metal ions on catalysis of the hammerhead, hepatitis delta virus, and group II intron ribozymes. Comparison of the observed cleavage rates of hammerhead nbozymes with the metal ion affinities of different ligands reveals that these rates correlate perfectly with the intrinsic phosphate affinities of the metal ions involved.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Chemistry
Dewey Decimal Classification:540 Chemistry
Language:English
Date:2010
Deposited On:23 Feb 2011 18:03
Last Modified:13 Nov 2016 06:49
Publisher:Elsevier
ISSN:1367-5931
Publisher DOI:https://doi.org/10.1016/j.cbpa.2009.11.024
PubMed ID:20047851
Other Identification Number:ISI:000277110800021

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