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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-46872

Eiwegger, T; Gruber, S; Geiger, C; Mayer, E; Dehlink, E; Bannert , C; Frischer, T; Kasper, D; Jaksch, P; Klepetko, W; Akdis, C A; Szépfalusi, Z (2011). Impact of systemic immuno-suppression after solid organ transplantation on allergen-specific responses. Allergy, 66(2):271-278.

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Abstract

INTRODUCTION:   The immunosuppressive therapy in solid organ transplantation targets mainly the T- and B-cell-mediated immune response. However, there is evidence that it neither suppresses sensitization nor clinical manifestation of allergic diseases in organ-transplanted patients. OBJECTIVE:   This study addresses the question whether allergen-specific responses are altered by systemic immunosuppression via negative effects on the T-regulatory cell compartment and a more pronounced suppression on Th1-type T-cell responses. MATERIAL AND METHODS:   Peripheral blood mononuclear cells from 65 solid organ-transplanted (kidney, liver, lung) children, adolescents, and young adults and 18 healthy, matched controls were included, and their clinical and sensitization status assessed. Allergen-specific proliferation, intracellular cytokine production, frequency of forkhead box P3 (FOXP3)+ CD3+ CD4+ CD25(high) cells, mRNA expression of IL-10, transforming growth factor (TGF)-β and FOXP3 (real-time RT-PCR) of peripheral blood mononuclear cells or bronchoalveolar lavage fluid (BAL)-derived cells, and the inhibitory capacity of T-reg cells were investigated. RESULTS:   Immunosuppression led to a significantly altered regulatory marker profile expressed by enhanced TGF-β mRNA production and a reduced frequency of FOXP3+ CD4+ CD3+ cells in solid organ transplanted individuals. FOXP3 expression in BAL cells of lung-transplanted patients was significantly decreased. Allergen-specific proliferation was not significantly altered despite long-term immunosuppression. However, suppression of allergen-specific responses via the T-regulatory cell fraction was deficient in immunosuppressed individuals. CONCLUSION:   The results suggest an insufficient control of allergen-specific responses via the Treg-cell compartment under systemic immunosuppression.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Swiss Institute of Allergy and Asthma Research
DDC:610 Medicine & health
Language:English
Date:February 2011
Deposited On:08 Mar 2011 15:47
Last Modified:22 Oct 2014 11:12
Publisher:Wiley-Blackwell
ISSN:0105-4538
Publisher DOI:10.1111/j.1398-9995.2010.02475.x.
PubMed ID:21208218

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