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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-47193

Cardona, A; Saalfeld, S; Preibisch, S; Schmid, B; Cheng, A; Pulokas, J; Tomancak, P; Hartenstein, V (2010). An integrated micro- and macroarchitectural analysis of the drosophila brain by computer-assisted serial section electron microscopy. PLoS Biology, 8(10):e1000502.

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Abstract

The analysis of microcircuitry (the connectivity at the level of individual neuronal processes and synapses), which is indispensable for our understanding of brain function, is based on serial transmission electron microscopy (TEM) or one of its modern variants. Due to technical limitations, most previous studies that used serial TEM recorded relatively small stacks of individual neurons. As a result, our knowledge of microcircuitry in any nervous system is very limited. We applied the software package TrakEM2 to reconstruct neuronal microcircuitry from TEM sections of a small brain, the early larval brain of Drosophila melanogaster. TrakEM2 enables us to embed the analysis of the TEM image volumes at the microcircuit level into a light microscopically derived neuro-anatomical framework, by registering confocal stacks containing sparsely labeled neural structures with the TEM image volume. We imaged two sets of serial TEM sections of the Drosophila first instar larval brain neuropile and one ventral nerve cord segment, and here report our first results pertaining to Drosophila brain microcircuitry. Terminal neurites fall into a small number of generic classes termed globular, varicose, axiform, and dendritiform. Globular and varicose neurites have large diameter segments that carry almost exclusively presynaptic sites. Dendritiform neurites are thin, highly branched processes that are almost exclusively postsynaptic. Due to the high branching density of dendritiform fibers and the fact that synapses are polyadic, neurites are highly interconnected even within small neuropile volumes. We describe the network motifs most frequently encountered in the Drosophila neuropile. Our study introduces an approach towards a comprehensive anatomical reconstruction of neuronal microcircuitry and delivers microcircuitry comparisons between vertebrate and insect neuropile.

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Neuroinformatics
DDC:570 Life sciences; biology
Language:English
Date:1 January 2010
Deposited On:04 Mar 2011 16:06
Last Modified:27 Nov 2013 22:00
Publisher:Public Library of Science
Series Name:PLoS biology
Number of Pages:0
ISSN:1544-9173
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:10.1371/journal.pbio.1000502
Citations:Web of Science®. Times Cited: 48
Google Scholar™
Scopus®. Citation Count: 76

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