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Structure of the recombinant antibody Fab fragment f3p4


Frey, D; Huber, T; Plückthun, A; Grütter, M G (2008). Structure of the recombinant antibody Fab fragment f3p4. Acta Crystallographica. Section D, Biological Crystallography, 64(Pt 6):636-643.

Abstract

The structure of the antibody Fab fragment f3p4, which was selected from a subset of the synthetic HuCAL antibody library to bind the sodium citrate symporter CitS, is described at 1.92 A resolution. Comparison with computational models revealed deviations in a few framework positions and in the binding loops. The crystals belong to space group P2(1)2(1)2 and contain four molecules in the asymmetric unit, with unit-cell parameters a=102.77, b=185.92, c=102.97 A. These particular unit-cell parameters allowed pseudo-merohedral twinning; interestingly, the twinning law relates a twofold screw axis to a twofold axis.

The structure of the antibody Fab fragment f3p4, which was selected from a subset of the synthetic HuCAL antibody library to bind the sodium citrate symporter CitS, is described at 1.92 A resolution. Comparison with computational models revealed deviations in a few framework positions and in the binding loops. The crystals belong to space group P2(1)2(1)2 and contain four molecules in the asymmetric unit, with unit-cell parameters a=102.77, b=185.92, c=102.97 A. These particular unit-cell parameters allowed pseudo-merohedral twinning; interestingly, the twinning law relates a twofold screw axis to a twofold axis.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Department of Biochemistry
07 Faculty of Science > Department of Biochemistry
Dewey Decimal Classification:570 Life sciences; biology
Language:English
Date:June 2008
Deposited On:04 Nov 2008 09:16
Last Modified:05 Apr 2016 12:31
Publisher:International Union of Crystallography
ISSN:0907-4449
Publisher DOI:10.1107/S0907444908007282
PubMed ID:18560151
Permanent URL: http://doi.org/10.5167/uzh-4811

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