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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-48375

Blumer, S; Greub, G; Waldvogel, A; Hässig, M; Thoma, R; Tschuor, A; Pospischil, A; Borel, N (2011). Waddlia, Parachlamydia and Chlamydiaceae in bovine abortion. Veterinary Microbiology, 152(3-4):385-393.

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Abstract

The etiology remains unknown in many cases of bovine abortion in Switzerland. Bacteria of the Chlamydiales order are known abortive agents, therefore cases of bovine abortion from three representative regions of Switzerland were investigated in this study. Particularly Chlamydiaceae as well as the Chlamydia-like organisms Waddlia and Parachlamydia were of interest, especially because of their possible zoonotic potential. Placenta samples (n=343) were tested for these bacteria by different PCR-methods, immunohistochemistry and serology for Chlamydia abortus. Additionally an attempt for the isolation of Waddlia and Parachlamydia was made by co-cultivation in amoebae. In 67.3% of the 343 cases a necrotizing and/or purulent placentitis was found histologically. By real-time PCR, 0.9% (3/343) of the cases were positive for Waddlia, 13.4% (46/343) positive for Parachlamydia and 14.6% (50/343) positive or questionable positive for Chlamydiaceae. Of these samples, confirmation by immunohistochemistry was possible in 2/3 cases for Waddlia, 25/46 for Parachlamydia and 4/50 for Chlamydiaceae. Of the 50 cases positive or questionable positive for Chlamydiaceae, species-identification by ArrayTube Microarray or 16S rRNA PCR resulted in 41 cases positive for C. abortus whereas the presence of Chlamydia suis was confirmed in four and Chlamydia pecorum in one case. This study brought evidence for the importance of different members of Chlamydiales in different regions of Switzerland although Waddlia is not occurring in a high prevalence. On the other hand mixed infections with different Chlamydiales as well as with other abortigenic agents could be found.

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Veterinary Pathology
05 Vetsuisse Faculty > Veterinary Clinic > Department of Farm Animals > Division of Herd Health
DDC:570 Life sciences; biology
630 Agriculture
Uncontrolled Keywords:Bovine abortion, Chlamydiaceae, Parachlamydia, Waddlia
Language:English
Date:2011
Deposited On:16 Jun 2011 12:16
Last Modified:27 Nov 2013 23:30
Publisher:Elsevier
ISSN:0378-1135
Publisher DOI:10.1016/j.vetmic.2011.05.024
PubMed ID:21658867
Citations:Web of Science®. Times Cited: 14
Google Scholar™
Scopus®. Citation Count: 13

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