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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-48712

Schnyder, M; Tanner, I; Webster, P; Barutzki, D; Deplazes, P (2011). An ELISA for sensitive and specific detection of circulating antigen of Angiostrongylus vasorum in serum samples of naturally and experimentally infected dogs. Veterinary Parasitology, 179(1-3):152-158.

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Abstract

Canine angiostrongylosis is an emerging cardiopulmonary disease in Europe which can be
fatal if left untreated. We developed a sandwich-ELISA based on a monoclonal antibody
(mAb Av 56/1/2) and on polyclonal rabbit antibodies directed against Angiostrongylus vasorum
adult excretory/secretory – antigen for the detection of circulating serum antigen of A.
vasorum. The sensitivity of the test was 95.7% (78.1–99.9, 95% CI) as determined with sera
of 23 dogs naturally infected with A. vasorum. The specificity was 94.0% (83.5–98.7, 95%
CI) using 50 dog sera (control group) submitted for reasons other than parasitic infections.
Potential cross-reactions were investigated with sera of a group of totally 61 dogs with
proven infections with Dirofilaria immitis (n = 23), Crenosoma vulpis (n = 14), Ancylostoma
caninum (n=4) or Toxocara canis (n = 20). No significant difference was observed concerning
the proportion of positive reactions between the control group and the group with
proven helminth infections other than A. vasorum. In experimentally inoculated dogs with
proven worm burdens of A. vasorum, the proportion of seropositive dogs increased over
the first 3 months of infection, starting from 35 days post inoculation (dpi) which was
before the onset of larval excretion. Ten weeks post inoculation, 98.6% of the dogs were
seropositive, and circulating antigen persisted in two dogs with long-term follow-up over
286 and 356 days, respectively. In contrast, in dogs with a single treatment with imidacloprid/
moxidectin at four or 32 dpi, no circulating antigen was observed, while in dogs
treated at 88–92 dpi, OD values decreased within 13–34 days. The specific detection of
circulating A. vasorum antigen by ELISA represents a valid alternative for reliable diagnosis
and for follow-up investigations after anthelmintic treatment. Moreover, the test can be
used for mass screening in large epidemiological investigations.

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Parasitology
04 Faculty of Medicine > Institute of Parasitology
DDC:570 Life sciences; biology
610 Medicine & health
600 Technology
Language:English
Date:2011
Deposited On:19 Jul 2011 10:40
Last Modified:27 Nov 2013 20:59
Publisher:Elsevier
ISSN:0304-4017
Publisher DOI:10.1016/j.vetpar.2011.01.054
PubMed ID:21333451
Citations:Web of Science®. Times Cited: 16
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